一株蜡状芽孢杆菌产蛋白酶脱毛性能研究  

Study on the Unhairing Properties of Proteinases Produced by a Bacillus Cereus

作　　者：陈倩[1] 关泽文[1] 刘彦[1] 

机构地区：[1]皮革化学与工程教育部重点实验室,四川大学制革清洁技术国家工程实验室,四川成都610065

出　　处：《皮革科学与工程》2013年第5期10-14,共5页Leather Science and Engineering

基　　金：国家863项目"工业酶分子改造与绿色生物工艺"(2012AA022200)

摘　　要：酶脱毛由于其无公害,对环境污染小且能回收高品质的毛必将成为制革工业清洁化生产的重要发展方向。本课题通过对一株蜡状芽孢杆菌发酵产生的蛋白酶(命名为EK-20)进行脱毛性能研究,发现该酶具有良好的脱毛效果。转鼓脱毛和堆置脱毛较适宜的pH为7.6,温度为36℃,酶浓度分别为200 U/g,250 U/g。与AS.1398蛋白酶脱毛对比实验表明:EK-20酶脱毛速度略慢于AS.1398蛋白酶,但脱毛后的裸皮洁白柔软,粒纹清晰;蓝湿皮丰满色正,手感良好;成革经摔软后未发现粒面损伤。经测试蓝湿皮的各项物理性能指标均达到或超过了行业的相关标准。Enzymetic unhairing is less pollution, meanwhile the by-product-hair has a high quality and can be recycled, so enzymatic unhairing is friendly to environment and will play an important role in clean leather production. The enzymes ob- tained by fermentation of a Bacilllus subtilis mutant No.307 named EK-20 was applied to the unhairing of goat skins. Experi- ments showed that the optimal pH and temperature of the enzyme action is 7.6, 36 ℃,.respectively. The optimal concentration of drum and painting enzymatic unhairing is 200 U/g, 250 U/g respectively. The results indicated that the unhairing rate of EK-20 was slower than that of AS.1398 proteinase, and the pelts were still white, soft and silkly, and no damage to the grain, as compared with AS. 1398 proteinase. The physical properties testing showed that the shrinkage temperature of the wet blue are above 100℃. The tensile strength and elongation at break both meet national standards of goat garment leather. As a po- tential unhairing enzyme, EK-20 may be used in the clean production of leather making in the future.

关 键 词：蛋白酶 脱毛 山羊皮 

分 类 号：TS54[轻工技术与工程—皮革化学与工程]