尖孢镰刀菌碳化硅量子点标记及其长时程荧光成像  

Labeling Fusarium oxysporum with silicon carbide quantum dots and long-term-distance fluorescent imaging for living cells

作　　者：宋月鹏[1,2] 康杰[1,2] 高东升 李江涛[3] 王晓波[4] 尹承苗 毛志泉 

机构地区：[1]山东农业大学机械与电子工程学院,泰安271018 [2]山东省园艺机械与装备重点实验室,泰安271018 [3]中国科学院理化技术研究所,北京100190 [4]山东文登整骨医院,文登264400

出　　处：《农业工程学报》2013年第17期286-292,共7页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金(51001111);"十二五"国家支撑计划项目(2011BAD12B02);山东省博士后创新基金(201203101);山东省研究生教育创新计划(SDYY11079);中国博士后科学基金(2013M531636)

摘　　要：致病性尖孢镰刀菌(Fusarium oxysporum)的荧光标记与活体细胞的长时程荧光成像示踪是研究其侵染机制较为有效的方法之一。采用化学腐蚀法制备出SiC量子点标记材料,研究了化学腐蚀法制备工艺过程,对SiC量子点标记材料的微观结构及光学性能进行检测分析,而后对尖孢镰刀菌进行标记及长时程荧光成像,结果表明,SiC量子点具有良好的生物相容性及光学性能,发射光颜色与其尺寸有内在关联性,腐蚀过程中即可在表面形成多种亲有机物功能团。尖孢镰刀菌生长初期SiC量子点将标记在分生孢子细胞膜处,而后通过内吞作用,进入活体细胞内部并形成稳定标记。进一步研究发现,SiC量子点无细胞毒性,可以实现活体细胞的长时程荧光成像,同时对其标记特征及原理进行了初步分析讨论。这对于尖孢镰刀菌侵染致病过程组织学与细胞学特征研究,揭示其致病机理、发展新型植株枯萎防治方法提供直观依据和理论分析基础。Fluorescence microscopy has allowed the functional study of various molecules that have been identified in living cells. The capabilities of this technique have generated a huge interest in developing new probes for labeling molecules and observing changes in their cellular activities. Quantum dots （QDs） based on II-VI （e.g., CdSe, CdTe, CdS, and ZnSe） and III-V （e.g., InP and InAs） semiconductors have attracted considerable scientific interest over the past two decades for their remarkable luminescent properties. However, the widely used CdX semiconductor QDs were found to be cytotoxic through the release of free metallic ions （cadmium ions for instance）. Therefore, it becomes clear that the cytotoxicity strongly influencing biological cell functioning is one of the major limiting factors for the application of II-VI QDs in efficient living cell imaging. Recently, silicon carbide（SiC） QDs has been growing attention for people with the advantages of non-toxic, good biocompatible due to its simple preparation process and excellent optical properties. Especially, for the Fusarium oxysporum, inspection of its infection mechanism needs to be fluorescent marked and long-term-distance tracing with vivo cells. The paper focus on two aspects for SiC QDs： fabrication and the vivo cells marking and long-term-distance fluorescent imaging. Firstly, the aqueous SiC QDs was prepared by SiC particles, which was prepared via self-propagating combustion in the mixture chemical etchant of nitric and hydrofluoric acid. Latticed-hollow of silicon carbide particles will be easily obtained because of SiC particles fabrication characteristics like nonequilibrium thermal forming leading to many defaults on the surface. Processed by broken and dispersed with ultrasonic cavitation, high-speed centrifugation, non-cytotoxic labeling materials of silicon carbide QDs were obtained. The microstructure and optical properties of QDs were measured and analyzed. The silicon carbide QDs are nearly spherical feature and its dia

关 键 词：荧光 标记 成像技术 长时程荧光成像 尖孢镰刀菌 活体细胞 碳化硅量子点 

分 类 号：O657.3[理学—分析化学]