胚胎干细胞视网膜下移植实验研究初步报告  

A preliminary report on experimental studies of embryonic stem cells transplanted into subretinal space

作　　者：唐仕波[1] 邱观婷[1] 李加青[1] 卢凤荷[1] 

机构地区：[1]中山医科大学中山眼科中心,广州510060

出　　处：《中华眼底病杂志》2000年第4期253-256,共4页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金!资肋项目 (39770 790 ;30 0 70 80 3);广东省自然科学基金!资助项目 (950 32 9)

摘　　要：目的　探讨胚胎干细胞 (embronic stem cell,ESC)在视网膜下间隙分化发育的情况。　方法　ESC经体外悬浮培养 4d形成胚胎体 (embryonic body,EB)备作移植。将 ESC以及 EB联合或不联合视黄酸(retinoic acid,RA)分别移植到 SD大鼠玻璃体腔或视网膜下 ,在视网膜下间隙移植眼 ,于移植前结扎眼动脉40 s造成缺血再灌注损伤的实验模型。于移植后 14～ 2 8d摘除眼球 ,观察移植物分化发育的情况。　结果　 ESC在 SD大鼠玻璃体腔内迅速增生 ,形成非典型性增生团块 ;EB在缺血再灌注损伤眼视网膜下间隙经RA诱导能定向分化为视网膜感光细胞 ;而在无 RA作用下向多极化分化发育。单独 RA视网膜下间隙注射后导致神经视网膜上皮增生变厚。　结论　ESC源性的 EB在缺血再灌注眼视网膜下间隙经 RA诱导能向视网膜感光细胞分化。Purpose [WT5”BZ]To investigate the development of embryonic stem cells (ESC)in the subretinal space. [WT5”HZ]Methods [WT5”BZ]ESC were cultivated in suspension for 4 days till they developed into cell aggregates,i.e.embryonic body(EB).ESC as well as EB combined with or without RA were respectively transplanted into vitreous cavity and subretina1 space in SD rats,and the subretinal transplanted eyes,transient ischemia reperfusion injuries were made by ligating the ophthalmic artery for 40 seconds before the transplantation .The experimental eyes were enucleated for histological and immunohistochemical assays after 14～28 d. [WT5”HZ]Results [WT5”BZ]The EB was found to develope into photoreceptors induced by RA in the subretinal space under an ischemia reperfusion condition,and EB transplantation without RA induction induced multiple differentiations in the subretinal space.The single injection of RA without EB induced hyperplasia of the neural retinal cells.ESC transplanted into vitreous cavity rapidly proliferated and developed into atypical hyperplastic mass. [WT5”HZ]Conclusion EB derived from ESC can differentiate into photoreceptors induced by RA in the host subretinal space under an ischemia reperfusion condition. [WT5”HZ]

关 键 词：胚胎干细胞移植 视网膜变性 ESC EB 

分 类 号：R774.1[医药卫生—眼科]