人大肠癌HT-29原位移植瘤裸小鼠模型建立方法的比较  

Comparison of the Methods for the Establishment of Human Colorectal Cancer HT-29 In-situ Transplantable Tumor Model in Nude Mice

作　　者：朱琰[1] 刘静[2] 史勤[2] 吕祥[2] 

机构地区：[1]上海中医药大学附属曙光医院药剂科,上海200021 [2]上海市中医医院肿瘤科,上海200071

出　　处：《中国药房》2013年第37期3463-3465,共3页China Pharmacy

基　　金：上海市自然科学基金项目(No.08ZR1419300);上海市教育委员会科研创新项目(No.08YZ59)

摘　　要：目的:比较组织块包埋法和组织块生物胶粘贴法建立人大肠癌HT-29原位移植瘤裸小鼠模型的特点和差异。方法:将24只BALB/c裸小鼠均分为两组,分别采用组织块包埋法(包埋法组)和组织块生物胶粘贴法(粘贴法组)建立人大肠癌HT-29原位移植瘤模型,于接种后8周处死,考察两组裸小鼠建模前后的体质量和成瘤率、瘤质量、瘤体积,以及发生肿瘤转移和产生腹水的情况。结果:与粘贴法组比较,包埋法组裸小鼠的瘤质量更大(P<0.05),建模前后体质量和瘤体积间差异无统计学意义(P>0.05),两组裸小鼠的成瘤率均为100%。粘贴法组只有4只裸小鼠发生肿瘤转移,1只产生腹水;包埋法组所有裸小鼠均发生肿瘤转移,有6只产生腹水。结论:两种方法建模均表现出良好的成瘤率和肿瘤生长,但包埋法组能更好地表现肿瘤转移等生物学特征。OBJECTIVE： To compare the characteristics and difference of tissue mass investment method and tissue mass bio- gel glue method to establish human colorectal cancer HT-29 in-situ transplantable tumor model in nude mice. METHODS： 24 BALB/c nude mice were divided into 2 groups. The human colorectal cancer HT-29 in-situ transplantable tumor model was estab- lished by tissue mass investment method （investment group） and tissue mass biogel glue method （glue group） separately. All nude mice were sacrificed after 8 weeks. The changes of body weight, tumor developing rate, tumor weight, tumor volume, tumor me- tastasis and ascites were observed in 2 groups before and after modeling. RESULTS： Compared with glue group, tumor weight of nude mice in the investment group were greatly changed （P〈0.05） ; there was no statistical significance in the difference of body weight and tumor weight before and after modeling （P〉0.05）. The tumor developing rates of both groups were 100%. 4 nude mice suffered from tumor metastasis and 1 suffered from ascites in glue group; all mice suffered from tumor metastasis and 6 suf- fered from ascites in investment group. CONCLUSIONS： Both methods can show sound tumor developing rate and tumor growth, but the investment group shows better tumor biocharacteristics such as metastasis.

关 键 词：大肠癌 HT-29原位移植瘤 裸小鼠 组织块包埋法 组织块生物胶粘贴法 建模 

分 类 号：R965[医药卫生—药理学]