布地奈德对哮喘模型大鼠气道炎症的缓解作用及其机制研究  

Remission Effects and Mechanism of Budesonide on Airway Inflammation in Asthmatic Rats

作　　者：王苑[1] 王优[2] 

机构地区：[1]广东医学院附属医院药学部,广东湛江524001 [2]广东医学院附属医院儿科,广东湛江524001

出　　处：《中国药房》2013年第37期3470-3472,共3页China Pharmacy

摘　　要：目的:研究布地奈德对哮喘模型大鼠气道炎症的缓解作用及其机制。方法:取大鼠随机分为正常对照组、模型组和低、中、高剂量布地奈德(0.25、1、2 mg/kg)组,每组10只,除正常对照组外其余各组大鼠以卵白蛋白(OVA)致敏激发法制备哮喘模型;布地奈德组于第14天开始雾化吸入OVA前30 min雾化吸入相应剂量的布地奈德溶液进行干预,每次10 min,每日2次,连续7d。末次雾化24 h后尾静脉取血和支气管肺泡灌洗留取肺泡灌洗液(BALF),检测血清和BALF中嗜酸粒细胞(Eos)、白细胞介素4(IL-4)、免疫球蛋白E(IgE)及胸腺活化调节趋化因子(TARC)的水平。结果:与正常对照组比较,模型组和低剂量布地奈德组大鼠血清和BALF中Eos数量和IL-4、IgE和TARC的含量均明显增加(P<0.05),中、高剂量布地奈德组间比较上述指标差异无统计学意义(P>0.05);与模型组比较,中、高剂量布地奈德组大鼠血清和BALF中Eos数量和IL-4、IgE、TARC含量均明显降低(P<0.05),低剂量布地奈德组上述指标差异无统计学意义(P>0.05)。结论:布地奈德能缓解哮喘模型大鼠的气道炎症,且与剂量呈正相关;其可能与降低TARC含量有关。OBJECTIVE： To study the remission effect and mechanism of budesonide on airway inflammation in asthmatic rats. METHODS： Rats were randomly divided into normal control group, model group and budesonide low-dose, medium-dose and high-dose groups （0.25, 1, 2 mg/kg） with 10 rats in each group. The asthmatic model was established in those groups by the oval- bumin （OVA） provocation methods, except for normal control group. Budesonide group was given aerosol inhalation of budesonide solution for 10 rain each time, twice a day for consecutive 7 days, 30 min before aerosol inhalation of OVA since the 14th day. Blood and bronchoalveolar lavage fluid （BALF） were collected 24 h after the last inhaling budesonide. The levels of eosinophilic granulocyte （Eos）, IL-4, IgE and thymus and activation regulated chemokine （TARC） in serum and BALF were measured. RE- SULTS： Compared with normal control group, the number of Eos, the contents of IL-4, IgE and TARC in serum and BALF were increased significantly in model group and budesonide low-dose group （P〈0.05） ; there was no statistical significance in above in- dex of budesonide medium-dose and high-dose groups （P〉0.05）. Compared with model group, the number of Eos, the contents of IL-4, IgE and TARC in serum and BALF of budesonide medium-dose and high-dose groups were decreased significantly （P〈 0.05）; there was no statistical significance in above index of budesonide low-dose group （P：〉0.05）. CONCLUSIONS： Budesonide can relieve airway inflammation in asthmatic rats in dose-dependant manner, which may be associated with the decrease of TARC.

关 键 词：胸腺活化调节趋化因子 布地奈德 哮喘模型 大鼠 气道炎症 缓解作用 机制 

分 类 号：R965[医药卫生—药理学]