HPLC法测定地蒽酚水溶蜡棒的含量  

Content Determination of Dithranol Water-soluble Wax Sticks by HPLC

作　　者：闫军[1] 刘燕[2] 张清[2] 李伟[2] 李柳[2] 王海钠[2] 

机构地区：[1]济南市皮肤病防治院,济南250001 [2]山东大学药学院,济南250012

出　　处：《中国药房》2013年第37期3519-3520,共2页China Pharmacy

基　　金：济南市科技发展计划资助项目(No.201010004)

摘　　要：目的：建立测定地蒽酚水溶蜡棒含量的方法。方法：采用高效液相色谱法。色谱柱为ZORBAX Eclipse XDB-C8,流动相为乙腈-水（60∶40）,流速为1.0 ml/min,检测波长为288 nm,进样量为20μl,柱温为25℃。结果：地蒽酚检测质量浓度线性范围为59.64~139.16μg/m（lr=0.999 8）,平均回收率为99.91%,RSD=0.57%（n=9）。结论：建立的含量测定方法灵敏度高、结果准确、重复性好,可用于地蒽酚水溶蜡棒的含量测定。OBJECTIVE： To develop a method for the content determination of Dithranol water-soluble wax sticks. METH- ODS： HPLC method was adopted. The determination was performed on ZORBAX Eclipse XDB-C8 column with mobile phase con- sisted of acetonitrile-water （60：40） at the flow rate of 1.0 ml/min. The detection wavelength was 288 nm and the column tempera- ture was set at 25 ℃. The sample size was 20 p.1. RESULTS： The linear range of dithranol was 59.64-139.16 μg/ml （r=-0.999 8） with an average recovery of 99.91% （RSD=0.57%, n=9）. CONCLUSIONS： The method is sensitive, accurate and reproduc- ible, and it is applicable for content determination of Dithranol water-soluble wax sticks.

关 键 词：地蒽酚水溶蜡棒 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]