酸性染料比色法测定咳喘口服溶液中盐酸麻黄碱的含量方法改进  

Improvement of the Method for Content Determination of Ephedrine Hydrochloride in Kechuan Oral Solu tion by Acid Dye Colorimetry

作　　者：李文静[1] 徐进[1] 景霞[1] 初立梅[1] 

机构地区：[1]南京医科大学附属南京儿童医院药学部,南京210008

出　　处：《中国药房》2013年第37期3534-3536,共3页China Pharmacy

摘　　要：目的：对酸性染料比色法测定咳喘口服溶液中盐酸麻黄碱的含量测定方法进行改进。方法：分别考察了酸性染料（溴麝香草酚蓝）溶液pH值为5.0、5.8和6.5,提取试剂为三氯甲烷、乙酸乙酯、乙醚和二氯甲烷-乙酸乙酯（50∶50）时对盐酸麻黄碱吸光度的影响,在此基础上考察了酸性染料和提取试剂的用量,并优化了反应用具和吸收波长;同时与原方法比较测定结果。结果：最终确定的优化后的反应条件如下：3 ml的样品溶液加入4 ml酸性染料溶液（pH 5.8）,经4 ml三氯甲烷提取后,在414 nm波长处测定吸光度。盐酸麻黄碱检测质量浓度线性范围为5.00~20.00μg/m（lr=0.998 3）,低、中、高3个质量浓度的回收率均大于98%（RSD=1.78%,n=9）;2种方法测得的盐酸麻黄碱的平均含量差别不大,但是改进后方法与原方法的RSD分别为0.15%~0.55%、1.45%~3.25%。结论：改进后的含量测定方法准确度好、重现性好,操作简便、快速,可用于咳喘口服溶液中盐酸麻黄碱的含量测定。OBJECTIVE： To improve acid dye colorimetry for content determination of ephedrine hydrochloride in Kechuan oral solution. METHODS： The effects of acid dyes （bromothymol blue） solution （pH 5.0, 5.8 and 6.5） and extract solution of chloroform, ethyl acetate, diethyl ether and dichloromethane-ethyl acetate （50：50） on ephedrine hydrochloride were investigated. The volume of acid dye and extract solution were investigated, and the instruments and absorption wavelength were optimized on the basis of pre-research. The results of optimized method was compared with previous method. RESULTS ： The optimized method was as follows： 3 ml sample solution mixed with 4 ml volume acid dyes solution （pH 5.8） ; after extracted with 4 ml chloroform, the absorbancy was determined at 414 nm .The linear range of ephedrine hydrochloride was 5.00-20.00 I.tg/ml （r=0.998 3）. The re- coveries of method were over 98% （RSD= 1.78%, n=9） at low, medium and high concentrations. There was no significant differ- ence in the contents of ephedrine hydrochloride by 2 kinds of methods. RSD of optimized method and previous method were 0.15 %-0.55 % and 1.45 %-3.25 %, respectively. CONCLUSIONS： The optimized method is accurate, reproducible, simple and rap- id, which is suitable for the content determination of ephedrine hydrochloride in Kechuan oral solution.

关 键 词：咳喘口服溶液 盐酸麻黄碱 酸性染料比色法 含量测定 改进 

分 类 号：R927.2[医药卫生—药学]