1，3-丁二烯作业工人外周血淋巴细胞遗传物质损伤水平的研究  

Genetic damage in peripheral blood lymphocyte of 1,3-butadiene workers

作　　者：刘楠[1] 王学生[1] 孟会林[1] 崔涛[2] 程娟[2] 肖经纬[2] 李忠生[2] 李斌[2] 

机构地区：[1]河北联合大学公共卫生学院河北省煤矿卫生与安全实验室,唐山063000 [2]中国疾病预防控制中心职业卫生与中毒控制所

出　　处：《卫生研究》2013年第5期754-757,共4页Journal of Hygiene Research

基　　金：国家自然科学基金(No.30471471);唐山市科技支撑计划项目(No.131302972)

摘　　要：目的评价1，3-丁二烯（BD）作业工人外周血淋巴细胞遗传物质的损伤。方法收集个人职业史、年龄、性别、吸烟和饮酒状况等信息，气相色谱法检测作业环境的BD浓度，应用外周血淋巴细胞胞质分裂阻滞微核（CBMN）检测法和彗星试验，评价180名BD作业工人和58名非职业暴露对照人群外周血淋巴细胞染色体和DNA损伤水平。结果作业区空气中BD浓度为1．80（0．59～2．76）mg／m^3。BD作业工人外周血淋巴细胞微核率（CBMN）、核质桥率（NPB）、核芽率（NBUD）和OliveTM分另IJ为（6．76±4．99）％o、1．00（0．00—4．00）、2．00（0．00～7．00）和4．64（3．50～5．98），均高于非暴露组（3．10±2．65）‰、0．00（0．00～2．80）、1．00（0．00～5．00）和2．34（0．82～3．93），差异有统计学意义（P〈0．01）。按BD作业工龄将180名BD作业工人分为3组：1～年、14-年和20～年组，校正了年龄、性别、吸烟和饮酒后，BD作业工人CBMN率有随作业工龄的增加而增加的趋势。结论在现有BD暴露水平下，外周血淋巴细胞胞质分裂阻滞微核法和彗星试验均能检出BD作业工人的遗传物质损伤，并有随作业工龄的延长而增加的趋势。Objective To investigate the DNA and chromosome damage in peripheral blood lymphocyte of workers occupationally exposed to 1,3-butadiene （BD）. Methods Personal information including occupational history, age, sex, smoking and drinking status was collected by the questionnaire. Gas chromatography was used to analyze the BD level. One hundred and eighty 1,3-butadiene workers and 58 controls without occupational BD exposure were investigated. Comet assay and cytokinesis-block micronucleus（CBMN） detection were used to evaluate DNA and chromosomal damage levels in peripheral blood lymphocyte. Results The concentration of BD in the working environment of BD-exposed workers was 1.80 （0.59 -2.76） mg/m3. The rate of CBMN, NPB, NBUD and Olive TM of lymphocyte in BD-exposed workers [ （ 6.76 ±4.99 ） %0, 1.00 （ 0. 00 - 4. O0 ） , 2. 00 （0.00 -7.00） and 4.64（3.50 -5.98）, respectively] were higher than those in controls [ （3.10 ±2.65）%0, 0. 00（0. 00 -2. 80）, 1.00（0. 00 -5.00） and 2.34（0. 82 -3.93）, P 〈 0.011. According to the length of work, 180 BD-exposed workers were classified into 3 groups： 1 yrs-, 14.0 yrs- and 20. 0 yrs- group, respectively after adjusting the age,sex, smoking and drinking, the rate of CBMN was a rising tendency along with the increase in length of work. Conclusion Under present BD exposure levels, both comet assay and Cytokinesis-block mieronucleus test could detect BD-indueed genotoxieity in BD-exposed workers, and are more suitable to assess the cumulative damage effect on DNA.

关 键 词：1 3-丁二烯 职业暴露 彗星试验 胞质分裂 阻滞微核试验 

分 类 号：R135.1[医药卫生—劳动卫生] Q343.1[医药卫生—公共卫生与预防医学]