亚慢性砷暴露小鼠体内不同形态砷的分布及对DNA的损伤作用  

Distribution of arsenic species and its DNA damage in subchronic arsenite-exposed mice

作　　者：李晗君[1] 林婧[2] 李玉锋[1] 严金婷[3] 李柏[1] 张维 董泽琴 陈春英[5] 

机构地区：[1]中国科学院高能物理研究所多学科研究中心,北京100049 [2]福州大学食品安全分析与检测教育部重点实验室 [3]国家人口计生委科学技术研究所 [4]贵州省环境科学研究设计院 [5]国家纳米科学中心

出　　处：《卫生研究》2013年第5期764-769,776,共7页Journal of Hygiene Research

基　　金：欧盟第六框架计划项目(FOOD-CT-2006-016253);贵州省科技厅社会发展攻关计划项目(黔科合[2010]3008)

摘　　要：目的探讨亚慢性砷暴露小鼠体内各器官组织不同形态砷的分布情况及砷对6种其他元素在机体内平衡的影响,同时研究亚慢性砷暴露对DNA损伤的影响。方法将8周龄C57BL/6N小鼠按体重匹配分为2组,分别给予0、10μg/ml亚砷酸钠溶液通过其饮用水摄入,持续暴露6个月。结果亚慢性砷暴露后,砷在各组织器官都有明显的蓄积,肾脏、肺和肝的含量较高,分别达到(563.9±222.5)、(458.6±191.0)和(279.8±81.2)ng/g,血液和脑组织中较低,分别为(82.2±26.7)ng/ml和(101.8±30.1)ng/g。染毒组小鼠的各个器官组织中砷主要以二甲基胂酸(DMA)形式存在。与对照组相比,染毒组部分器官中Cr、Cu、Zn、Se、Pb的含量存在显著性差异(P<0.05),Cd含量无显著性差异。染毒组的8-OHdG含量为(149.1±1.0)ng/ml,明显高于对照组的(76.4±27.9)ng/ml。结论小鼠经亚慢性砷暴露,导致砷在各组织器官中均有分布,且主要以二甲基胂酸形式存在,同时砷也对Cr、Cu、Zn、Se、Pb在机体内的平衡产生影响,并对机体产生一定DNA损伤。Objective To study the arsenic distribution, speciation, its effects on the balance of other elements and the DNA damage by subchronic arsenite exposure in mice. Methods The 8-week-old C57BL/6N mice were matched by weight and divided into control group and supplementation group, which were given 0 or 10 μg/ml of sodium arsenite in the drinking water, and continuous exposed for 6 months. Results Arsenic was found in various tissues and organs. The highest ones were in the kidney, lung and liver, reached （563.9 ± 222.5）, （458.6 ± 191.0） and （279.8 ± 81.2） ng/g,respectively while the lowest in the blood and brain, reached （82.2 ± 26.7） ng/ml and （101.8 ± 30. 1 ） ng/g, respectively. Arsenic exists mainly in the form of dimethylarsinous acid （DMA）. Compared to the control group, there was a significant difference （P 〈 0.05） between arsenic and chromium, copper, zinc, selenium, lead in some organs of arsenic exposed group, but not cadmium. Furthermore, the 8-hydroxydeoxyguanosine （8- OHdG） level of the exposed group was （ 149. 1 ±1.0） ng/ml, which was significantly higher than the control group of （76.4 ±27.9） ng/ml. Conclusion Arsenic accumulated in various tissues and organs mainly in the form of DMA, which affected the balance of chromium, copper, zinc, selenium and lead in the body, and led to DNA damage after subchronic exposure.

关 键 词：亚慢性 砷 小鼠 八羟基脱氧鸟苷 

分 类 号：R599[医药卫生—内科学] R994.6[医药卫生—临床医学]