GLP-1基因诱导脐血内皮祖细胞VEGF和SDF-1/CXCR4表达  

Expression of VEGF and SDF-1/CXCR4 in cord blood endothelial progenitor cells induced by GLP-1 gene

作　　者：刘峰[1] 汤佳珍[1] 朱凌燕[1] 甘华侠[1] 

机构地区：[1]南昌大学第一附属医院内分泌科,江西南昌330006

出　　处：《中国现代医学杂志》2013年第23期32-36,共5页China Journal of Modern Medicine

基　　金：江西省自然科学青年基金(No:20114BAB215004).

摘　　要：目的探讨胰高血糖素样肽1(GLP-1)基因对人脐血内皮祖细胞(EPCs)中血管内皮生长因子(vascular endothelial growth factor,VEGF)和基质细胞衍生因子(stromal cell derived factor,SDF-1)/趋化因子受体4(chemokine receptor 4,CXCR4)信号通路的表达调控。方法构建GLP-1过表达载体转染EPCs,并分别以空白对照,0.3、0.5和0.8μg载体转染培养的EPCs。转染后的细胞用实时荧光定量PCR检测GLP-1的转染效果,并用实时荧光定量PCR和Western blotting检测VEGF、SDF-1和CXCR4的mRNA和蛋白表达水平。结果在不同浓度过表达的EPCs细胞中,0.5μg和0.8μg载体转染效果较好。实时定量PCR显示VEGF和SDF-1在加入0.5μg载体后的表达量最高,与对照组比较差异有统计学意义(P<0.05)。而CXCR4在加入0.5μg和0.8μg载体后的mRNA表达量均明显高于对照组(P<0.05)。Western blotting结果显示,VEGF、SDF-1和CXCR4的蛋白水平在转染GLP-1过表达载体后均出现不同程度的表达上调。结论 GLP-1能够诱导EPCs中VEGF和SDF-1/CXCR4的表达。GLP-1可通过调节VEGF和SDF-1/CXCR4促进EPCs增殖、分化和迁移。【Objective】 To discuss the influence of GLP1 genes＇ expression-regulation on VEGF and SDF-1/CXCR4 in EPCs.【Methods】 After construction of GLP-1 expression vector and transfection of EPCs,the vectors were transfected into EPCs in blank control,0.3 μg,0.5 μg and 0.8 μg concentration.The transfection effects were determined by RT-PCR.The mRNA and protein expression of VEGF,SDF-1 and CXCR4 were assayed by RT-PCR and western blotting.【Results】 In different vectors concentration,0.5 μg and 0.8 μg concentration of vectors showed better effects of transfection.RT-PCR showed that VEGF and SDF-1 had the highest mRNA expression after 0.5 μg vector transfection compared with control group（P 0.05）.CXCR4 mRNA expression were the highest in 0.5 μg and 0.8 μg compared with control group（P 0.05）.The result of western blotting showed that the protein expression of VEGF,SDF-1 and CXCR4 had different up-regulation after transfection.【Conclusions】 GLP-1 could induce expression of VEGF and SDF-1/CXCR4 in cord blood endothelial progenitor cells.GLP-1 could promote proliferation,differentiation,and migration of EPCs by up-regulation of VEGF and SDF-1/CXCR4.

关 键 词：胰高血糖素样肽1 内皮祖细胞 基质细胞衍生因子 血管内皮生长因子 趋化因子基质细胞衍生因子-1 

分 类 号：R587.1[医药卫生—内分泌] Q813[医药卫生—内科学]