白皮杉醇与牛血清白蛋白相互作用的研究  

Interaction between piceatannol and bovine serum albumin by fluorescence spectroscopy

作　　者：曾春姣[1] 罗亚[1] 彭慧[1] 刘芝兰[1] 

机构地区：[1]湘潭职业技术学院,湖南湘潭411104

出　　处：《中南药学》2013年第9期641-644,共4页Central South Pharmacy

摘　　要：目的应用荧光光谱法和紫外光谱技术对白皮杉醇(piceatannol)与牛血清白蛋白(BSA)之间的相互作用进行研究。方法计算白皮杉醇与BSA的结合常数K a、结合位点数及热力学函数△G、△S、△H,分析白皮杉醇对BSA的荧光淬灭过程及其与BSA的相互作用类型;运用Foerster’s偶极-偶极非辐射能量转移原理测定了白皮杉醇与BSA的结合距离。结果随着白皮杉醇浓度升高,BSA内源荧光强度有规律的下降;随温度的升高,静态淬灭常数和结合常数均下降;热力学函数△G<0、△S>0、△H<0。结论白皮杉醇对BSA的荧光淬灭属于自发的以疏水作用力为主导的非辐射能量转移的静态淬灭过程。Objective To study the interaction between bovine serum albumin （BSA） and piceatannol by fluorescence spectroscopy. Methods The respective binding constant Ka and number of binding sites ofpiceatannol as well as thermodynamic function A G, A S, and A H were calculated. The binding distance between piceatannol and BSA was obtained by Foerster＇s dipile-dipile non-radiation energy transfer mechanism. Results The fluorescence intensity of BSA decreased regularly upon addition ofpiceatannol. The static quenching constants and binding sites all decreased with increasing temperature. The thermodynamic fimction of A S was higher than 0 while both of A G and A H were lower than 0. Conclusion The binding between piceatannol and BSA is spontaneous, mainly dominated by hydrophobic forces, and occurs by non-radiation energy transfer.

关 键 词：白皮杉醇 牛血清白蛋白 荧光光谱法 

分 类 号：R966[医药卫生—药理学]