高效液相色谱法同时测定维血宁中虎杖苷、大黄素-8-O-β-D-葡萄糖苷、大黄素、大黄素甲醚的含量  

HPLC simultaneous determination of polydatin, emodin-8-O-β-D-glycosidase, emodin and physcion in Weixuening

作　　者：程茜菲[1] 常青[1] 刘琦[1] 许苗苗[1] 王珊[1] 宋小妹[1] 

机构地区：[1]陕西中医学院,陕西咸阳712046

出　　处：《中南药学》2013年第9期686-689,共4页Central South Pharmacy

基　　金：陕西省重大科技创新项目(No.2009ZKC-05)

摘　　要：目的建立雏血宁中虎杖苷、大黄素-8-O-β-D-葡萄糖苷、大黄素、大黄素甲醚4种化学成分的含量测定的HPLC方法。方法采用Kromasil C18（4．6mm×250mm，5p,m）色谱柱；0．1％的磷酸溶液和乙腈进行梯度洗脱；柱温30℃；流速1mL·min^-1；检测波长223nm。结果在HPLC法中，虎杖苷、大黄素-8-O-β-D-葡萄糖苷、大黄素、大黄素甲醚的线性范围分别是：0．009～0．072mg·mL^-1（r=0．9991）、0．048～0．386mg·mL^-1（r=1．0000）、0．027～0．214mg·mL^-1（r=1．0000）、0．024～0．188mg·mL^-1（，=1．0000）；平均回收率分别为98．8％、97．1％、95．5％、96．8％，RSD分别为0．6％、0．4％、0．3％、0．4％。结论所建立的方法专属性强，重复性好，可用于维血宁的质量控制。Objective To establish an HPLC method for the simultaneous determination of polydatin, emodin-8-O-β-D- glycosidase, emodin and physcion in Weixuening. Methods The chromatograhic separation was performed on Kromasil C18 column （4.6 mm × 250 mm, 5 μm）. The column temperature was 30 ℃. A linear elution of 0.1% phosphoric acid aqueous solu- tion-acetonitrile was adopted at the flow rate of 1 mL . min^-1. The UV detection wavelength was 223 nm. Results Polydatin, emodin-8-O-β-D-glycosidase, emodin and physcion had good linearity at 0.009 -- 0.072 mg .mL ^- 1（r = 0.999 1）, 0.048 -- 0.386 mg . mL^ -1 （r= 1.000 0）, 0.027 -- 0.214 mg .mL^-1 （r = 1.000 0）, and 0.024-0.188 mg . mL^- 1 （r = 1.000 0）, respectively. The average recoveries and RSD were 98.8% （0.6%）, 97.1% （0.4%）, 95.5% （0.3%）, and 96.8% （0.4%）, respectively. Conclusion This method is simple, accurate and reproduceable, which can be used for the quality control of Weixuening.

关 键 词：维血宁 虎杖苷 大黄素 大黄素甲醚 大黄素-8-O-β-D葡萄糖苷 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]