紫扇贝、海湾扇贝及其正反杂交子代群体遗传结构的AFLP分析  

AFLP analysis of genetic structure of Argopecten purpuratus,Argopecten irradians and their positive and negative hybrid offspring

作　　者：孙妍[1] 黄晓婷[1] 胡丽萍 王师[1] 王春德[2] 包振民[1] 

机构地区：[1]海洋生物遗传学与育种教育部重点实验室,中国海洋大学海洋生命学院,山东青岛266003 [2]青岛农业大学海洋科学与工程学院,山东青岛266109

出　　处：《海洋科学》2013年第8期1-5,共5页Marine Sciences

基　　金：国家高技术研究发展计划(2012AA10A410);国家自然科学基金项目(31172404);国家科技支撑计划项目(2011BAD45B01);现代农业产业技术体系建设专项资金资助项目(CARS-48)

摘　　要：本研究应用AFLP分子标记技术,对紫扇贝(Argopecten purpuratu,PP)、海湾扇贝(Argopecten irradians,Ⅱ)、正交子一代(紫扇贝♀×海湾扇贝♂,PI)及反交子一代(海湾扇贝♀×紫扇贝♂,PⅠ)群体的遗传结构进行了分析和比较。用5对AFLP引物扩增得到433个位点,其中多态位点为88.45%;正反交子代中的扩增位点大部分(97.23%)来源于紫扇贝和海湾扇贝;扩增中出现了非孟德尔遗传位点,如紫扇贝和海湾扇贝的部分扩增位点在杂交子代中丢失,正反交子代中出现12个紫扇贝和海湾扇贝中没有的扩增位点;通过多态位点比率、Nei’s基因多样性、香农式多样性指数、遗传距离和AMOVA分析发现,正反杂交子代之间的遗传分化最小,与紫扇贝和海湾扇贝的遗传分化均显著。The AFLP molecular marker technique was used to analyze the genetic structure of Argopecten purpu- ratus （PP）, Argopecten. irradians （II） and their hybrid offspring （A. purpuratus ♀× A. irradians ♂, PI; A. irradian3. ♀× A. purpuratus ♂, IP） in order to reveal the genetic differences and relationships between the parents and their offspring. Five AFLP primers were used to amplify the genome DNA, and 433 loci were yielded, the polymorphic loci of which accoutered for 88.45%. The majority of the amplified loci （97.23%） of the hybrid offspring were in- herited from both the parents, indicating that the hybrids were real heterogenous. Loci which not follow the Mende- lian Law were found in the analysis that some of the loci of the A. purpuratus and A. irradians were lost and 12 new loci were amplified in the hybrid offspring. It was revealed from the analysis of the percentage of polymorphic loci, Nei＇s gene diversity, Shannon＇s diversity index, genetic distance and AMOVA that the genetic differentiation be- tween the positisve and negative hybrid offspring was smaller than that between the offspring with their parents.

关 键 词：紫扇贝（Argopecten purpuratu） 海湾扇贝（Argopecten irradians） 杂交 AFLP 

分 类 号：S917.4[农业科学—水产科学]