金花茶体细胞胚胎Cn-SERK基因的克隆及其生物信息学分析  

Cloning and Bioinformatics Analysis of Cn-SERK Gene from Somatic Embryos in Camellia nitidissima Chi.

作　　者：翁浩[1] 赖钟雄[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所.福建福州350002

出　　处：《热带作物学报》2013年第9期1699-1707,共9页Chinese Journal of Tropical Crops

基　　金：福建省重大科技平台建设项目(No.2008N2001);国家科技支撑计划项目(No.2007BAD07B01)

摘　　要：以金花茶体细胞胚胎为材料,采用RT-PCR和RACE技术,获得了含有完整开放阅读框的金花茶CnSERK基因cDNA全长序列和DNA全长序列。Cn-SERK基因编码1个含有624个氨基酸的蛋白质。生物信息学分析显示该蛋白质为亲水、且具有信号肽的跨膜蛋白,定位于内质网的可能性最高。二级结构主要有α螺旋和无规则卷曲构成,存在1个蛋白激酶位点。此蛋白可能发生磷酸化的位点有25个。内含子分析结果表明,CnSERK基因由11个外显子和10个内含子组成,内含子的剪切位点符合真核生物GT-AG规则。系统进化树分析结果表明,金花茶Cn-SERK蛋白与毛果杨(Populus trichocarpa)的SERK亲缘关系较近。The full-length Cn-SERK cDNA and genomic DNA sequences were obtained from somatic embryos using RT-PCR and RACE techniques in Camellia nitidissima. Chi. The cDNA sequence encoded a polypeptide of 624 amino acids. Bioinformatics analysis showed that the protein was a hydrophilic protein, located in endoplasmic reticulum, with a signal peptide. The secondary structure was mainly constituted by the a-helix and random coil, and there existed a protein kinase signature and 25 phosphorylation sites. The Cn-SERK genomic DNA sequence contained 11 exons and 10 introns. The cleavage site of the introns conformed to the GT-AG rule of the eukaryotes. Phylogenetic analysis also indicated that the Cn-SERK was close to SERK of Populus trichocarpa in genetic relationship.

关 键 词：金花茶 体细胞胚胎 Cn—SERK 基因克隆 生物信息学 

分 类 号：S68[农业科学—观赏园艺] Q78[农业科学—园艺学]