无芒稗质体ACCase基因Real-timePCR定量方法的建立  

Establishment of Real-time PCR Assay for Quantitative of Plastid ACCase in Beardless Barnyardgrass

作　　者：郇志博[1] 王金信[2] 

机构地区：[1]中国热带农业科学院分析测试中心.海南海口571101 [2]山东农业大学植物保护学院.山东泰安271017

出　　处：《热带作物学报》2013年第9期1708-1713,共6页Chinese Journal of Tropical Crops

基　　金：高等学校博士学科点专项科研基金(No.20093702110003);国家自然科学基金(No.31171866、31201529);公益性行业(农业)科研专项(No.201303031)

摘　　要：为研究一种抗精喹禾灵无芒稗的抗药性机理,建立了一种以β-actin为内参基因利用实时荧光定量PCR技术测定抗性无芒稗质体ACCase基因相对表达水平的方法。分析结果表明,设计的实时定量引物特异性非常好,内参基因、目的基因的扩增效率分别为103.2%、104.3%,线性相关系数分别为0.993 0、0.991 2,完全满足Real-time PCR定量方法的要求。所建立的无芒稗质体型ACCase基因Real-time PCR定量方法将为研究质体型ACCase基因的表达和杂草对ACCase抑制剂的抗药性机制研究提供方法学参考。In order to study the resistance mechanism of a quizalofop-ethyl-resistance beardless barnyardgrass, a relative quantitative analysis method was established based on real-time PCR technology and β-actin gene as the housekeeping gene. The results showed the specificity of the two sets of primers was very well. The amplification efficiencies of the primers for ^-actin and plastid ACCase gene was 103.2% and 104.3, while the correlation coefficients was 0.993 0 and 0.991 2, respectively. The results showed the real-time PCR quantitative method established in the article could he provided as a methodological reference for the study of the expression of plastid ACCase gene and the study of the mechanism of weed resistant to ACCase inhibitors.

关 键 词：无芒稗 质体ACCase 基因表达 Real—time PCR 

分 类 号：Q33[生物学—遗传学]