千层塔全草总黄酮提取工艺的优化  

Optimization of Extraction of Total Flavone from Huperzia serrata

作　　者：武芸[1,2] 郑小江[1,2] 杨婷[2] 吴娟[2] 杨兰[2] 张泽[3] 武玉莲[3] 

机构地区：[1]生物资源保护与利用湖北省重点实验室,湖北民族学院,湖北恩施445000 [2]湖北民族学院生物科学与技术学院,湖北恩施445000 [3]湖北民族学院附属民大医院,湖北恩施445000

出　　处：《热带作物学报》2013年第9期1814-1818,共5页Chinese Journal of Tropical Crops

基　　金：生物资源保护与利用湖北省重点实验室开放课题(No.PKLHB1301);恩施州科技研究与开发2013年项目;国家级大学生创新创业训练计划项目(No.201210517030);获得湖北民族学院林学一级学科和食品科学与工程学科的资助

摘　　要：为了优化千层塔全草中总黄酮的提取工艺。采用索氏提取方法,以总黄酮提取率为考察指标,通过单因素试验确定各因素对提取工艺的影响,正交试验确定最佳提取工艺。结果表明:影响千层塔总黄酮提取效率的主次因素依次为:乙醇浓度>温度>提取时间>料液比。千层塔中总黄酮最佳提取工艺为:乙醇浓度60%、提取时间130 min、提取温度85℃、料液比(g/mL)1∶35,千层塔中总黄酮提取率为1.262 6%。The optimization of extraction of total fiavonoids from Huperzixt serrata was studied in this paper. The total flavonoids were extracted by Soxhlet extraction and the optimal extraction conditions were achieved by the orthogonal experiment. Experimental results showed that the factors infuencing the extracting total flavonoids were in the order： ethanol concentration 〉 extraction temperature 〉 extraction time 〉 solid-liqnid ratio. The optimization of extraction of total flavonoids was 60% alcohol concentration, extraction temperature 85 ~C, extraction time 130 min and solid-liquid ratio（g/mL）l：35. Under the condition, the extraction rate of total favonoids was 1.262 6%.

关 键 词：千层塔 总黄酮 正交试验 提取工艺 

分 类 号：Q914.85[天文地球—古生物学与地层学]