应用芪参益气滴丸治疗心肌病的效应与机制研究(英文)  

Effect and mechanism of Qishen Yiqi Pills on adriamycininduced cardiomyopathy in mice

作　　者：童嘉毅[1] 徐艳娟[1] 卞叶萍[2] 沈祥波[1] 闫磊[1] 朱欣佚[3] 

机构地区：[1]东南大学附属中大医院心内科,南京210009 [2]江苏省省级机关医院ICU,南京210009 [3]东南大学附属中大医院中医科,南京210009

出　　处：《中国天然药物》2013年第5期514-518,共5页

基　　金：supported by the National Natural Science Foundation of China(No.81070265)~~

摘　　要：目的:通过构建小鼠阿霉素心肌病模型,观察芪参益气滴丸对小鼠心肌病的治疗作用,并初步探讨其机制。方法:取健康雄性昆明小鼠64只,分为1、阿霉素组(ADR组,16只):生理盐水1 mL/100 g/d灌胃,ADR 4 mg·kg 1腹腔注射,每周两次,连续4周。2、阿霉素+芪参益气滴丸○1组(16只):ADR 4 mg·kg 1腹腔注射,每周两次,连续4周,第三周开始予芪参益气滴丸3.5 mg/100 g/d,灌胃给药,累计4周。3、阿霉素+芪参益气滴丸○2组(16只):ADR 4 mg·kg 1腹腔注射,每周两次,连续4周,同时予芪参益气滴丸3.5 mg/100 g/d,灌胃给药。4、对照组(16只):生理盐水1 mL/100 g/d灌胃,同时生理盐水1mL·kg 1腹腔注射,每周两次,累计4周。实验第六周末测定小鼠心功能,制作病理HE切片,用光镜行形态学观察,western法检测小鼠Bcl-2、Bax蛋白的表达。结果:1、ADR组小鼠LVESD、LVEDD增大,LVEF明显降低,芪参益气滴丸给药两组小鼠心功能较ADR组均有改善,组间对比有统计学差异(P<0.05)。2、病理结果显示ADR组心肌细胞变性水肿、排列紊乱,用药○1组及○2组病理结果均有不同程度的改善。3、Bcl-2蛋白表达阿霉素组<用药○1组<用药○2组<对照组,Bax蛋白表达阿霉素组>用药○1组>用药○2组>对照组,组间对比有统计学意义(P<0.05)。结论:芪参益气滴丸可有效改善阿霉素心肌病小鼠的心功能,纠正心衰,减轻心肌损害作用,且早期用药效果佳,其机制与抑制小鼠心肌细胞凋亡有关。AIM： To study the effect and probable mechanism of Qishen Yiqi Pills on adriamycin （ADR）-induced cardiomyopathy in mice. METHODS： Sixty-four mice were randomly divided into （1） the ADR group： saline （1 mL/100 g） administered every day by intragavage, ADR （4 mg.kg-1） administered to each mouse by intraperitoneal injection twice a week for four weeks; （2） the ADR ＋ Qishen Yiqi Pills I group： ADR （4 mg.kg-1） administered to each mouse by intraperitoneal injection twice a week for four weeks, and at the beginning of the third week Qishen Yiqi Pills （3.5 mg/100 g） administered by intragavage every day for four weeks; （3） the ADR ＋ Qishen Yiqi Pills II group： ADR （4 mg.kg-l） administered to each mouse by intraperitoneal injection twice a week for four weeks, and at the same time Qishen Yiqi Pills （3.5 mg/100 g） administered by intragavage every day for four weeks; （4） the control group： saline （1 mL/100 g） administered every day by intragavage, saline （1 mL.kg-t） administered to each mouse by intraperitoneal injection twice a week for four weeks. Six weeks later, cardiac function, myocardial pathology, and expression of Bcl-2 and Bax were evaluated. RESULTS： 1. The left ventricular diastolic diameter and the left ventricular systolic diameter were significantly increased （P 〈 0.05） and the left ventricular ejection fraction was significantly decreased （P 〈 0.05） in the ADR group, and the cardiac function of both the ADR ＋ Qishen Yiqi Pills I group and the ADR ＋ Qishen Yiqi Pills [I group improved. 2. Myocardial morphologic observation showed that the myocardial fibers were disordered, there was cell edema, and gap widening in the ADR group. The degree of myocar- dial cell injury was reduced in the ADR ＋ Qishen Yiqi Pills I group and ADR ＋ Qishen Yiqi Pills II group compared with the ADR group. 3. The expression of Bax in the ADR group was significantly up-regulated, and the expression of Bcl-2 was significantly down- regulated in

关 键 词：阿霉素心肌病 芪参益气滴丸 效应与机制 凋亡 

分 类 号：R965[医药卫生—药理学]