镰形棘豆提取物抗肝癌的体内外活性研究(英文)  

Antitumor effects of two extracts from Oxytropis falcata on hepatocellular carcinoma in vitro and in vivo

作　　者：杨光明[1] 燕茹[2] 王兆先[3] 张芳芳[4] 潘扬[1,5] 蔡宝昌[1] 

机构地区：[1]南京中医药大学江苏省中药炮制重点实验室 国家中医药管理局中药炮制标准重点研究室,南京210023 [2]澳门大学 [3]中国药科大学药学院,南京210009 [4]南京中医药大学江苏省中药炮制重点实验室国家中医药管理局中药炮制标准重点研究室,南京210023 [5]南京中医药大学药用菌与中药生物技术研究所,南京210023

出　　处：《中国天然药物》2013年第5期519-524,共6页

基　　金：supported by the National Natural Science Foundation of China(No.30902012)~~

摘　　要：目的:探讨镰形棘豆提取物的体内外抗肝癌活性,并探讨其可能的机制。方法:采用MTT法检测镰形棘豆提取物对SMMC-7721细胞的增殖抑制,采用PI单染法和Annexin V-FITC/PI双染法进行流式细胞仪检测,测定细胞周期和凋亡率,观察H22荷瘤小鼠的体内肿瘤抑制作用。结果:MTT实验表明,镰形棘豆提取物EOOF和FOF有效抑制肿瘤细胞SMMC-7721的增殖,并呈一定的剂量依赖性,其IC50值分别为0.115和0.097 mg·mL 1。TFOF作用SMMC-7721细胞后可使细胞周期停滞于G1期,凋亡细胞的比例升高,并且呈浓度依赖性。镰形棘豆提取物FOF能明显抑制H22荷瘤小鼠肿瘤的生长,低、高剂量组的抑制率分别为56.1%和70.8%(P<0.01)。结论:镰形棘豆提取物FOF能抑制SMMC-7721细胞的增殖,诱导其凋亡,并对H22荷瘤小鼠有肿瘤生长抑制作用。显示镰形棘豆具有较好的发展为抗肝癌药物的前景。AIMS： To investigate the antitumor effects of extracts from Oxytropis falcata on human hepatocellular carcinoma SMMC-7721 cells in vitro and in transplanted murine H22 tumors in vivo. METHODS： Cell proliferation, cell cycle distribution and apoptosis in SMMC-7721 cells were determined and tumor growth inhibition in H22 tumors was investigated. Cell cycle distribution was analyzed by flow cytometry with propidium iodide （PI） and Annexin V-FITC/ PI double staining. RESULTS： MTT assay revealed that essential oil and flavonoids of O. falcata （named EOOF and FOF） inhibited proliferation of SMMC-7721 cells in a dose-dependent manner. The IC50 value of EOOF and FOF were 0.115 and 0.097 mg.mL-1, respectively. Cell cycle was arrested at G1 phase, and induction of apoptosis occurred in SMMC-7721 cells when subjected to FOF. Growth inhibition in H22 solid tumors transplanted mice was significantly pronounced after being treated with FOF, and the inhibition ratio were 56.1% and 70.8% at the concentration of 30 and 60 mg.kg-1. CONCLUSION： The results suggest that FOF promotes apoptosis in SMMC-7721 cells and inhibits H22 tumor growth, resulting in a potential antitumor effect on hepatic cancer.

关 键 词：抗肿瘤 镰形棘豆 凋亡 SMMC-7721 H22荷瘤小鼠 增殖 

分 类 号：R965[医药卫生—药理学]