基于HPLC-DAD-FICL法建立虎杖的谱-效整合指纹图谱(英文)  

The Spectrum-Effect integrated fingerprint of Polygonum cuspidatum based on HPLC-diode array detection-flow injection-chemiluminescence

作　　者：丁晓萍[1] 张翠玲[1,2] 戚进[1,3] 孙丽琼[1] 秦民坚[4] 余伯阳[1,3] 

机构地区：[1]中国药科大学中药复方研究室,南京211198 [2]蚌埠医学院中药研究室,蚌埠233030 [3]天然药物活性物质与功能国家重点实验室,南京210009 [4]中国药科大学中药资源研究室,南京211198

出　　处：《中国天然药物》2013年第5期546-552,共7页

基　　金：supported by the National Natural Science Foundation of China(Grant No.30973965);46th,47th China Postdoctoral Fund(Nos.20090461139,20100471480);a grant from the Specialized Research Fund for the Doctoral Program of Higher Education of China(for the youth scholars)(No.20090096120008);2011’Program for Excellent Scientific and Technological Innovation Team of Jiangsu Higher Education;the Priority Academic Program Development of Jiangsu Higher Education Institutions,Postdoctoral Fund of Jiangsu Province(No.1001077C)~~

摘　　要：目的:建立虎杖药材的谱-效整合指纹图谱,为科学评价其质量提供可靠方法。方法:采用高效液相色谱-二极管阵列-流动注射化学发光法得到不同来源虎杖药材的谱-效整合指纹图谱,从而综合评价虎杖药材的质量。结果:对19批药材的化学指纹图谱和清除自由基的活性指纹图谱分别进行了相似度评价,并对其结果进行了对比分析。对13个主要抗氧化成分的色谱峰面积和活性进行了回归分析,并利用HPLC-ESI-MS鉴定了部分成分的化学结构。结论:基于高效液相色谱-二极管阵列-流动注射化学发光法所建立的虎杖的谱-效整合指纹图谱能快速筛选虎杖药材中的主要抗氧化活性成分,这种综合评价方法能更客观、有效地反映虎杖药材的质量。AIM： To establish the Spectrum-Effect integrated fingerprint of Polygonum cuspidatum to evaluate the quality of P. cuspidaturn. METHODS： An on-line HPLC-DAD-flow injection chemiluminescence （FICL） method was developed to investi- gate the quality of P. cuspidatum from different habitats based on the established Spectrum-Effect integrated fingerprint. RESULTS： Nineteen batches of samples of P. cuspidatum were evaluated for the similarity of their chromatographic and free radical scavenging fingerprints, and the results compared. Main antioxidants were estimated by regression analysis between peak areas of thirteen com- pounds and their activities. Some active compounds were identified by HPLC-ESI-MS. CONSULSIONS： The results indicated that main antioxidants in P. cuspidatum could be rapidly screened by the established Spectrum-Effect integrated fingerprint based on on-line HPLC-DAD-FICL, and would be more efficient and objective method to evaluate the quality of P. cuspidatum.

关 键 词：谱-效整合指纹图谱 高效液相色谱-二极管阵列-流动注射化学发光法 虎杖 高效液相-电喷雾-质谱 

分 类 号：R917[医药卫生—药物分析学]