猪源乳酸杆菌对致病性沙门菌、大肠埃希菌黏附仔猪小肠上皮细胞的影响  

Effect of Pig Lactobacillus to Salmonella,Escherichia coli Adhesion on Pig Small Intestinal Epithelial Cell

作　　者：凌晨[1] 蒋光邻[1] 钱莹[1] 宋康[1] 马燕[1] 王鹏雁[1] 蒋建军[1] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832000

出　　处：《西北农业学报》2013年第9期18-23,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：兵团博士基金项目(2009JC06);石河子大学"国家大学生创新性实验计划"项目(111075933)

摘　　要：为了通过仔猪小肠上皮细胞(ZYM-SIEC-02)体外培养模型,观察猪源乳酸杆菌对细胞的黏附作用,以及对致病性沙门菌、大肠埃希菌黏附细胞的竞争、排斥和置换作用。采用乳酸杆菌在体外与ZYM-SIEC-02共同孵育镜下观察其黏附效果;乳酸杆菌与致病性沙门菌、大肠埃希菌共同黏附ZYM-SIEC-02观察其黏附竞争性;用乳酸杆菌预处理ZYM-SIEC-02后再加入致病性沙门菌、大肠埃希菌孵育观察其黏附排斥性;用致病性沙门菌、大肠埃希菌预处理ZYM-SIEC-02后再加入乳酸杆菌孵育观察其黏附置换性。结果表明:乳酸杆菌可以黏附ZYM-SIEC-02,具有剂量效应。乳酸杆菌与致病性沙门菌或大肠埃希菌同时加入细胞一起培养时,能竞争性的黏附细胞且黏附抑制率也具有剂量效应。乳酸杆菌预处理细胞后再加入致病菌,高含量乳酸杆菌(1010 mL-1)能使致病菌的黏附率下降。但是,用致病菌预处理细胞后再加入乳酸杆菌,只有高含量的乳酸杆菌可以置换致病菌,而低含量乳酸杆菌却能增加致病菌对细胞的黏附。研究结果为乳酸菌作为微生态制剂用于临床上防治仔猪沙门菌病、大肠埃希菌病,减少食物源性污染和提高食品安全提供试验依据和理论基础。To evaluate the adhesion of pig Lactobacillus strains on Pig Small Intestinal Epithelial Cell line（ZYM SIEC 02）,the competition,exclusion and displacement of adhesion to Salmonella,Escherichia coli on ZYM SIEC 02 cells were investigated.The adhesion effect was observed when Lactobacillus were co cultivated with ZYM SIEC 02 cells;the adhesion competive was observed when Lactobacillus were co cultivated with Salmonella,Escherichia coli and ZYM SIEC 02 cells;after the ZYM SIEC 02 were pretreated by Lactobacillus, the adhesion exclusion was observed when Salmonella ,Escherichia coli were co cultivated with ZYM SIEC 02 cells; after the ZYM SIEC 02 were pretreated by Salmonella and Escherichia coli, the adhesion displacement was observed when Lactobacillus were co cultivated with ZYM SIEC 02 cells.The results show that the Lactobacillus exhibited dose dependent adhesion to ZYM SIEC 02.The ability to inhibit the competition adhesion of Salmonella or Escherichia coli appeared to depend on the dose by 1010 mL-1 Lactobacillus.Adhesion percent of Salmonella was reduced by pretreating the cells with 1010 mL-1 Lactobacillus prior to Lactobacillus adhesion to ZYM SIEC 02 cell monolayers.No displacement was detected at low concentration of Lactobacillus.These results would offered a new efficient way for preventing and theraping pig Salmonellosis,Escherichia coli，reducing food born pollution and raising foods safety.

关 键 词：乳酸杆菌 致病性沙门菌 致病性大肠埃希菌 仔猪小肠上皮细胞 黏附 

分 类 号：S852.61[农业科学—基础兽医学]