玉米种子萌发初期碱性α-半乳糖苷酶活性与种子脱水耐性的关系  

The Relevance between Alkalineα-Galactosidase Activity and Seed Desiccation Tolerance of Maize Seeds at Early Stage of Geremination

作　　者：张丁方[1] 关涛[1] 严俊[1] 韩庆辉[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《西北农业学报》2013年第9期48-54,共7页Acta Agriculturae Boreali-occidentalis Sinica

摘　　要：选取抗旱性不同的4个玉米自交系为试验材料,探究种子吸胀萌发过程中碱性α-半乳糖苷酶(AGA)活性与种子脱水耐性的潜在关系。结果表明,在不同吸胀时间抗旱品种吉853、E28与H21的AGA活性均高于干旱敏感品种B73。4个自交系的AGA活性在整个吸胀过程全都呈现高低起伏趋势,且在吸胀24h时均达到峰值。在吸胀不同时间后进行脱水处理(24h),再复水后,3个抗旱品种的种子活力、胚根与胚芽长都显著高于B73,且随脱水前吸胀时间的增加,种子活力、胚根与胚芽长均呈现逐渐降低的趋势。超氧阴离子含量在吸胀24h(并脱水处理)后达到峰值,同时种子活力降低至最低水平。相关分析表明,在种子萌发初期,抗旱玉米自交系吉853和E28的AGA活性与脱水耐性(种子活力、胚芽与胚根长)呈显著负相关(P<0.01)。Four maize inbred lines with different drought resistance were chosen to investigate the potential relevance between alkaline α Galactosidase activity and seed desiccation tolerance after imbibition. The results showed that drought tolerant lines Ji853, E28 and H21 had higher AGA activities than drought susceptible line B73 at almost all the imbibition time. AGA activities of these four inbred lines showed a trend of ups and downs at the whole geremination stage and AGA activities of these four inbred lines reached peak value when imbibition time was 24 h. Seed vigour, embryo length and radical length of drought tolerant lines Ji853, E28 and H21 were significant higher than B73 after imbibition, desiccation and rehydration and seed vigour, embryo length and radical length decreased gradually with the increase of imbibition time. O2· mass fraction reached peak value after imbibition for 24 h and desiccation for 24 h and seed vigour reached minimum at the same imbibition time. Correlation analysis showed AGA activity was significantly and negatively correlated with desiccation tolerance （seed vigour, embryo length and radical length） （P〈0.01）.

关 键 词：玉米 种子萌发 棉子糖系列寡糖 碱性α 半乳糖苷酶 脱水耐性 

分 类 号：S513[农业科学—作物学]