干旱胁迫对小蓬竹生理生化特性的影响  

Effect of Drought Stress on Physiological and Biochemical Characteristics in Drepanostachyum luodianense

作　　者：刘济明[1] 李鹏[1] 廖小锋[2] 赵晓鹏[1] 文萍[1] 池馨[1] 颜强[1] 

机构地区：[1]贵州大学林学院,贵阳550025 [2]贵州科学院贵州省山地资源研究所,贵阳550001

出　　处：《西北农业学报》2013年第9期153-157,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：贵州省国际科技合作计划项目[(2013)7010]

摘　　要：以小蓬竹幼苗为试验材料,采用人工控水的方法,设置田间持水量的60%(轻度干旱,LD)、40%(中度干旱,MD)、20%(重度干旱,SD)3个水分胁迫梯度,以田间持水量的80%为对照(CK),研究了小蓬竹生理生化特性对水分胁迫的响应情况。结果表明,经过70d水分胁迫试验,可溶性糖、可溶性蛋白及脯氨酸含量,在LD条件下呈上升趋势,MD、SD条件下,上升显著,且脯氨酸含量持续累积。随着干旱胁迫的增强,MDA含量缓慢下降,质膜透性变化不明显,SOD活性变化幅度较小,CAT活性表现为先升后降趋势,但是上升幅度较小,POD活性逐渐上升。研究认为,小蓬竹通过体内的生理生化机制,尤其通过增加渗透调节物质的含量及保护酶间的协调作用,以降低膜脂过氧化程度,实现对干旱胁迫较强的忍耐性和较好的适应性。Taken the seedlings of Drepanostachyum luodianense as the materials, the indexes of physiological and biochemical characteristics were analyzed under different water treatments, including 60%（low drought stress, LD）, 40%（moderate drought stress, MD）, 20%（severe drought stress, SD）of field capacity, and 80%（control, CK） of field capacity as the control. After 70 days of drought stress, the results showed that the osmotic substances including soluble sugar, soluble protein and proline presented ascending trend under LD, the ascending range was significant under LD or SD, and proline had obvious accumulation. With the increasing of drought stress, the content of MDA declined slowly, the plasma membrane of D. luodianense did not change obviously; the activities of SOD remains almost the same; the activities of POD rose gradually; the activity of CAT rose at first and lowered later, the increasing extent was relatively small. We concluded that D. luodianense could endure and adapt to drought stress in some extents through physiological and biochemical mechanisms, especially through increasing the content of osmotic substances and coordinating the activity of protective enzymes.

关 键 词：小蓬竹 干旱胁迫 MDA 保护酶活性 膜脂过氧化 

分 类 号：S795.9[农业科学—林木遗传育种]