固相萃取-高效液相色谱法同时测定泥炭沼泽源水体中9种酚类物质  

Determination of phenols from peatland water by solid phase extraction and high performance liquid chromatography

作　　者：万翔[1] 向武[1,2] 于桑[1] 邬钰[1] 智超[1] 

机构地区：[1]中国地质大学(武汉)地球科学学院,武汉430074 [2]生物地质与环境地质国家重点实验室,武汉430074

出　　处：《分析试验室》2013年第10期15-19,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(41172330)资助

摘　　要：建立了固相萃取-高效液相色谱同时分析测定泥炭沼泽区水体中原儿茶酸、对羟基苯甲酸、香草酸、龙胆酸、咖啡酸、阿魏酸、水杨酸、对香豆酸(trans-4-Hydroxycinnamic acid)、丁香酸等9种酚类的方法。沼泽区水体采用MEGA Bond Elut Plexa固相萃取柱富集后,在含5 g/L乙酸的V(甲醇):V(水)=5:95流动相中用Agilent ZORBAX SB-Aq色谱柱(250 mm×4.6 mm,5μm)分离,外标法定量。结果表明,9种酚类物质在0.1～60 mg/L范围内均呈良好的线性关系,线性相关系数均大于0.9987,仪器检出限0.07～0.37 mg/L。空白水中的加标回收率为90.4%～106.8%,相对标准偏差(n=6)为1.9%～6.2%。应用该方法测定了若尔盖泥炭沼泽区水体样品,9种酚酸均检出。A simple method based on solid phase extraction（ SPE） coupled with high performance liquid chromatography（ HPLC） was developed for the determination of protocatechuic acid,p-hydroxybenzoic acid,vanillic acid,gentisic acid,caffeic acid,ferulic acid,salicylic acid,trans-4-hydroxycinnamic acid and syringic acid in peat land water. The extraction of the water sample was performed on an MEGA Bond Elut Plexa solidphase extraction cartridge. The separation of the nine compounds was achieved on an Agilent ZORBAX SB-Aq column（ 250 mm × 4. 6 mm,5μm） with methanol-water（ 5： 95,V / V） containing 5 g / L acetic acid as mobile phase. The quantification was achieved by the external standard method. The results showed that good linearities were observed in the ranges of 0. 1 ～ 60 mg / L with the correlation coeffcients ≥0. 9987 and the instrumental limits of detection for the nine target compounds were in the range of 0. 07 ～ 0. 37 mg / L. The average recoveries of the nine target compounds were 90. 4% ～ 106. 8% and the relative standard deviations（ n = 6） were 1. 9% ～6. 2%. This method was applied to the analysis of the water samples from the Zoige peat land. The results showed that 9 kinds of phenolic compounds were found in the water from Zoige peat land water.

关 键 词：固相萃取 高效液相色谱法 酚酸 若尔盖沼泽水 

分 类 号：O657.7[理学—分析化学]