北细辛汉城细辛及华细辛中总马兜铃酸含量测定  

Determination of Total Aristolochic Acid in Three Kinds of Asarum

作　　者：于艳[1] 王晓荣[1] 魏新智[1] 孙科峰[1] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《辽宁中医药大学学报》2013年第10期44-45,共2页Journal of Liaoning University of Traditional Chinese Medicine

摘　　要：目的:测定北细辛、汉城细辛和华细辛中总马兜铃酸的含量,为其临床安全用药提供实验依据。方法:以马兜铃酸A为对照,采用紫外分光光度法,检测波长为390 nm。结果:马兜铃酸A浓度在3.0~30.0μg·mL^(-1)范围内与吸收度呈良好线性关系,Y=19.844X+0.0272(r=0.9996),平均回收率为97.84%,RSD为0.97%。三种细辛中总马兜铃酸的含量为0.17%~0.35%,以汉城细辛最低,华细辛最高。结论:本方法操作简便,重复性好,精密度高,可用于细辛中总马兜铃酸的含量测定。Objective ： To determine the content of total aristolochic acid in Asarum heterotropoides Fr.Var.mandshurium, Asarum sieboldii Miq.Var.eoulense Nakai and sarum Sieboldii Miq, and provide the basis for clinical application of asarum. Methods ： The ultraviolet spectrophotometry was used with aristoloehie acid A as the chemical reference.The UV detector wavelength was 390 nm. Results ： The content of aristolochic acid A showed a linear relation with the spectrophotometric absorbance within the range of 3.0 μg·mL-1to 30.0 μg·mL-1, and the linear equation was Y=19.844X＋0.0272 （ r=0.9996 ） .The average recoverT was 97.84%, and RSD was 0.97%.Their total aristolochic acid content varied from 0.17% to 0.35%.h was the lowest in Asarum sieboldii Miq.Var.eoulense Nakai and the highest in Asarum sieholdii Miq. Conclusion ： The method is simple, repeatable and suitable for determining the content of the total aristolochic acid in Asarum.

关 键 词：北细辛 汉城细辛 华细辛 总马兜铃酸 紫外分光光度法 

分 类 号：R284[医药卫生—中药学]