湘西盲高原鳅线粒体DNA遗传多样性分析  

Genetic diversity analysis of mitochondrial DNA in Triplophysa xiangxiensis

作　　者：姚雁鸿[1] 牟东[1] 汪登强[1] 孔令富[2] 何力[1] 何文辉[3] 

机构地区：[1]中国水产科学研究院长江水产研究所,湖北武汉430223 [2]云南农业大学动物科技学院,云南昆明650201 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《上海海洋大学学报》2013年第5期684-689,共6页Journal of Shanghai Ocean University

基　　金：国家科技支撑计划(2004BA526B10);国家科技部公益性行业专项(200903048)

摘　　要：对取自湖南湘西自治州龙山县乌龙山3个不同洞穴的湘阿卣高原鳅（Triplophysaxiangxiensis）群体82尾样本的线粒体细胞色素b（Cytb）基因序列和控制区（D—loop）序列进行了研究，分析了其遗传分化及遗传多样性。湘西占高原鳅线粒体Cytb堆W序列片段长1140bp，D—loop序列片段长934bp，在D—loop中发现6个多态位点且均为单一变异位点，D—loop序列中检测到7个啦倍体，其单倍型多样性指数（h）在0．083至0．282之间，其核苷酸多样性（pi）指数范嗣为0．00009～0．00032。3个采样群体的遗传多样性均较低。令部样本的Cytb序列均一致，小存在变异，4种碱基T、C、A、G含链分别是28．3％、28．6％、28．1％、15．0％，AT含量较高。D—loop的分子变异方差分析（AMOVA）结果显示湘西占高原鳅遗传变异系数Fst=-0．0089，总遗传变异中，各年度种群内变异为100．89％，年度种群问变异为-0．89％，变异主要来自年度种群内部。In this study, the genetic diversity and genetic differentiation of a total of 82 individuals from three Triplophysa xiangxiensis populations which were sampled from three caves in Wulongshan mountain were evaluated using mitochondrial DNA （mtDNA）, cytochrome b （Cytb） gene and control region （D-loop） sequences. 1 140 bp of the mitochondrial Cytb partial sequences and 934 bp of D-loop partial sequences were obtained. No genetic variation was found in cyt b, and all sequences were identical, the contents of T, C,A, and G nucleotides oecounted for 28.3% ,28.6% ,28.1% , 15.0%. In the control region,the sequence analysis of the 73 samples revealed 7 haplotypes with haplotype diversity ranging from 0. 083 to 0. 282.6 polymorphic sites were found from all samples, and all were singleton variables. Seven haplotypes were detected among the sequences,with one of them （haplotype 1 ） being popular which was represented by 73 individuals. As for annual samples, sequences of all individuals from year 2008 were completely identical except one, and four and five haplotypes were determined from specimens of year 2009 and 2010, respectively. The nucleotide diversisty ranged from 0.000 09 to 0. 000 32, respectively. All of which revealed that the genetic diversity in these three T. xiangxiensis populations was low. Analysis of molecular variance （AMOVA） revealed that 100.89% of the genetic variability occurred within annual temporary samples, and negative value of 0.89% came from among temporary samples. The network constructed by sequences of control region displayed a starlike topology, with the haplotype 1 in center and the other haplotypes connecting with it by one step of mutation.

关 键 词：湘西盲高原鳅 线粒体DNA 控制区 细胞色素B 遗传多样性 

分 类 号：S917[农业科学—水产科学]