不同剂量丙泊酚和异氟醚对豚鼠耳蜗血流的影响  

Effects of different doses of propofol or isoflurane on cochlear blood flow in guinea pigs

作　　者：肖颖[1] 田福宏[2] 温健[1] 王伟[1] 

机构地区：[1]西安交通大学第一附属医院麻醉科,710061 [2]陕西省韩城矿务局总医院麻醉科

出　　处：《中华麻醉学杂志》2013年第7期786-790,共5页Chinese Journal of Anesthesiology

摘　　要：目的 评价不同剂量丙泊酚和异氟醚对豚鼠耳蜗血流（COBF）的影响.方法 54只雄性豚鼠,3月龄,体重400 ～ 500 g,采用随机数字表法,将其分为6组（n=9）,P1组、P2组和P3组均经5min静脉注射丙泊酚负荷量5 mg/kg,随后分别以10、20、40 mg·kg-1 ·h-1速率输注115 min;S1组、S2组和S3组维持呼气末异氟醚浓度分别为1％、2％、3％,持续吸入120 min.分别于给药前（基础水平）和给药期间间断记录MAP和COBF.分别于给药前（T1）、停止给药时（T2）和停止给药后1 h（T3）记录畸变产物耳声发射（DPOAE）振幅.每组处死5只动物,取Corti器,扫描电镜下观察外毛细胞形态.结果 丙泊酚可降低MAP、升高COBF和DPOAE振幅,且呈剂量依赖性,而异氟醚可呈剂量依赖性降低MAP和COBF,1％异氟醚可使DPOAE振幅升高,2％和3％异氟醚可降低DPOAE振幅,且可导致外毛细胞损伤.结论 丙泊酚可升高豚鼠COBF,而高剂量异氟醚可降低COBF,其原因与抑制了其自动调节机制,使COBF更易受灌注压的影响,从而损伤耳功能.Objective To evaluate the effects of different doses of propofol or isoflurane on the cochlear blood flow （CBF） in guinea pigs.Methods Fifty-four adult male guinea pigs,aged 3 months,weighing 400-500 g,were randomly divided into 6 groups （n =9 each）.In P1,P2 and P3 groups,propofol was infused for 115 min at 10,20 and 40 mg· kg-1 · h-1,respectively,after a loading dose of 5 mg/kg was injected over 5 min.In S1,S2 and S3 groups,isoflurane was inhaled for 120 min with end-tidal concentrations of 1％,2％ and 3 ％,respectively.Mean arterial pressure and CBF were recorded before administration （baseline,T1） and during the period of drug administration.Distortion product otoacoustic emission （DPOAE） was measured at T1,at the end of administration （T2） and 1 h after administration （T3）.Five animals in each group were sacrificed and organs of Corti were harvested for observation of morphology of out hair cells by scanning electron microscopy.Results Propofol decreased MAP and increased CBF and DPOAE amplitude in a dose-dependent manner.Isoflurane decreased MAP and CBF in a dose-dependent manner.1％ isoflurane increased DPOAE amplitude,however,2％ and 3％ isoflurane decreased it and caused damage to out hair cells.Conclusion Propofol induces an increase in CBF in guinea pigs,while high concentration of isoflurane induces a decrease in CBF.Isoflurane inhibits CBF autoregulation,which makes CBF more sensitive to perfusion pressure,thus causing damage to hearing function.This is the reason why high concentration of isoflurane induces a decrease in CBF.

关 键 词：二异丙酚 异氟醚 耳蜗 局部血流 听觉 

分 类 号：R614[医药卫生—麻醉学]