异丙酚、依托咪酯和咪达唑仑对大鼠肾组织缝隙连接功能的影响  

Effects of propofol, etomidate and midazolam on gap junction function in rats

作　　者：罗晨芳[1] 原冬冬[1] 李晓芸[1] 黑子清[1] 

机构地区：[1]中山大学附属第三医院麻醉科,广州市510630

出　　处：《中华麻醉学杂志》2013年第7期791-794,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81170449）;广东省自然科学基金重点项目（S2011020002780）

摘　　要：目的 评价异丙酚、依托咪酯以及咪达唑仑对大鼠肾组织缝隙连接（GJ）功能的影响.方法 离体实验大鼠肾小管上皮细胞NRK52E,以1.0×105/ml密度接种于6孔培养板（2 ml/孔）,采用随机数字表法,将其分为4组（n=6）：对照组（C组）、异丙酚组（P组）、依托咪酯组（E组）和咪达唑仑组（M组）.C组采用含10％胎牛血清的DMEM-F12培养液培养,P组、E组和M组分别于培养液中加入异丙酚、依托咪酯和咪达唑仑,终浓度分别为15、8、4 μmol/L,孵育1h,采用细胞接种荧光示踪法测定GJ功能.在体实验健康SPF级雄性大鼠24只,2月龄,体重220 ～ 280 g,采用随机数字表法,将其分为4组（n=6）：异丙酚组（P组）、依托咪酯组（E组）和咪达唑仑组（M组）分别腹腔注射异丙酚、依托咪酯和咪达唑仑100、6和5 mg/kg,对照组（C组）给予等容量生理盐水,连续3d.最后1次给药后30 min时取肾皮质,采用在体荧光示踪法测定GJ功能.结果 离体与在体实验：与C组比较,P组和E组GJ功能降低（P＜0.05）,M组GJ功能差异无统计学意义（P＞0.05）.结论 异丙酚和依托咪酯可明显抑制NRK52E细胞及大鼠肾组织GJ功能,而咪达唑仑对其无影响.Objective To evaluate the effects of propofol,etomidate and midazolam on gap junction function in rats in vitro and in vivo.Methods Experiment Ⅰ NRK52E cells were seeded in 6-well plates with the density of 1.0 × 105/ml and randomly divided into 4 groups （n =6 wells each）：control group （group C）,propofol group （group P）,etomidate group （group E） and midazolam group （group M）.In group C,NRK52E cells were cultured in DMEM-F12 culture medium containing 10％ fetal bovine serum.In P,E and M groups,propofol,etomidate and midazolam （with the final concentrations of 15,8 and 4μmol/L,respectively） were added to the culture medium,respectively,and the cells were then incubated for 1 h.Parachute dye-coupling assay was used to measure the gap junction function in NRK52E cells.Experiment Ⅱ Twenty-four male Sprague-Dawley rats,aged 2 months,weighing 220-280 g,were randomly divided into 4 groups （n =6 each）：propofol group （group P）,etomidate group （group E）,midazolam group （group M）,and control group （group C）.In P,E and M groups,propofol 100 mg/kg,etomidate 6 mg/kg and midazolam 5 mg/kg were injected intraperitoneally once a day for 3 consecutive days,respectively.The equal volume of normal saline was given instead in group C.The animals were sacrificed at 30 min after the last administration and the renal cortex was harvested to measure the gap junction function using tissue scrape and load assay.Results Compared with group C,the gap junction function was significantly decreased in P and E groups （P ＜ 0.05）,and no significant change was found in the gap junction function in group M （P ＞ 0.05）.Conclusion Propofol and etomidate significantly inhibit the gap junction function in NRK52E cells and renal tissues in rats,but midazolam produces no effect.

关 键 词：二异丙酚 依托咪酯 咪达唑仑 缝隙接合部 肾 NRK52E细胞 

分 类 号：R614[医药卫生—麻醉学]