触液核在大鼠慢性瘙痒维持中的作用  

Role of cerebrospinal fluid-contacting nuclei in maintenance of chronic itch in rats

作　　者：宿世玉[1] 田恩琪[1] 党秀静[1] 孔敏[1] 张励才[1] 

机构地区：[1]徐州医学院麻醉学重点实验室,221002

出　　处：《中华麻醉学杂志》2013年第7期807-809,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30871307）;江苏省自然科学基金（BK2012580）

摘　　要：目的 探讨触液核在大鼠慢性瘙痒维持中的作用.方法 雄性SD大鼠,3月龄,体重240～ 280 g.第一部分采用随机数字表法,将42只大鼠分为3组（n=14）：生理盐水组（C组）、丙酮组（A组）和恶唑酮组（O组）.O组于颈背部涂抹0.5％恶唑酮15μl,C组和A组分别涂抹等容量生理盐水和丙酮,分别于给药后7、9、13、16、17、18、21、23 d重复上述处理,记录每次给药后30 min内搔抓次数,每组取6只动物,在最后1次涂抹恶唑酮后2h取脑组织,测定触液核c-Fos表达.第二部分 采用随机数字表法,将24只大鼠分为3组（n=8）：正常对照组（C1组）、慢性瘙痒组（CI组）和慢性瘙痒＋触液核毁损术组（CI＋ KA组）,CI组和CI＋ KA组采用上述方法制备慢性瘙痒模型,触液核毁损术于第8次给药后6h实施,并记录第9次给药后30 min内搔抓次数.结果 第一部分 与C组比较,A组T4-8时搔抓次数增多,O组T1-8时搔抓次数增多,A组和O组触液核c-Fos表达上调（P＜0.05）；与A组比较,O组T1-8时搔抓次数增多,触液核c-Fos表达上调（P＜0.05）.第二部分 与C1组比较,CI组和CI＋ KA组搔抓次数增多（P＜0.05）,而CI＋ KA组搔抓次数少于CI组（P＜0.05）.结论 触液核参与了大鼠慢性瘙痒的维持.Objective To evaluate the role of cerebrospinal fluid-contacting nuclei （CSF-CN） in maintenance of chronic itch in rats.Methods Forty-two adult male Sprague-Dawley rats,aged 3 months,weighing 240-280 g,were used in the study.The experiment was performed in two parts.Part Ⅰ Forty-two Sprague-Dawley rats were randomly divided into 3 groups （n =14 each）：control group （C group）,acetone group （A group） and oxazolone group （O group）.0.5％ oxazolone 15 μl was applied to the neck and back of rats in group O,while the equal volume of normal saline and acetone was applied in groups C and A,respectively.Application of the drug mentioned above was repeated on day 7,9,13,16,17,18,21 and 23 after the first stimulation in each rat in each group.Scratching behaviors were oberserved within 30 min after each stimulaiton.Six rats in each group were chosen and sacrificed after the last application of oxazolone,and the brains were obtained for determination of c-Fos expression in CSF-CN.Part Ⅱ Twenty-four rats were randomly divided into 3 groups （n =8 each）：control group （C1 group）,chronic itch group （group CI）,and chronic itch ＋ lesion group （CI ＋ KA group）.Chronic itch was induced by repeated application of oxazolone as previously described in CI and CI ＋ KA groups.The chemical lesion of CSF-CN was performed at 6 h after 8th application of the drug.Then the scratching behaviors were observed within 30 min after 9th application of the drug.Results Part Ⅰ Compared with C group,the scratching behaviors were increased significantly at T4-8 in A group,and at T1-8 in O group （P ＜ 0.05）,and the expression of c-Fos was up-regulated in O and A groups （P ＜ 0.05）.Compared with A group,the scratching behaviors were increased significantly at T1-8 and the expression of c-Fos was up-regulated in O group （P ＜ 0.05）.Part Ⅱ Compared with C1 group,the scratching behaviors were significantly increased in CI and CI ＋ KA groups （P ＜ 0.05）.The scratching behaviors were sig

关 键 词：神经元 瘙痒症 触液核 

分 类 号：R614[医药卫生—麻醉学]