基于D-loop区序列分析的四川省三个地方鸡种遗传多样性及母系亲缘关系研究  

Genetic Diversity and Maternal Relationship of Three Local Chicken Breeds from Minority Area of Sichuan Province Based on D-loop Rigion

作　　者：蒋利[1] 蒋小松[2] 熊斌[3] 王康环[1] 王海[1] 刘光伟[1] 任冰冰[1] 徐亚欧[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]四川省畜牧科学研究院,四川成都610066 [3]冕宁县畜牧局,四川凉山615600

出　　处：《中国家禽》2013年第18期10-14,共5页China Poultry

基　　金：四川省畜禽育种攻关项目(2011NZ0099-6);四川省应用基础项目(2013JY0044);西南民族大学研究生"创新型科研项目"(CX2013SZ57)

摘　　要：为研究四川省少数民族地区泸宁鸡、米易鸡、草科鸡的遗传多样性及母系亲缘关系,通过PCR扩增、产物回收、测序的方法,获得各鸡种30个样本的mtDNA D-loop区的部分序列,利用DNAMAN、MEGA5、DnaSP、Network等生物信息学软件对测序结果进行分析。对90个个体mtDNA D-loop区589 bp的片段通过数据分析及聚类分析,构建了单倍型网络关系图及品种聚类图等。泸宁鸡、米易鸡、草科鸡具有较近的亲缘关系,推测3个地方鸡种起源于分布在印度支那半岛、泰国、苏门答腊的红原鸡Gallus gallus gallus亚种。To investigate genetic diversity and maternal origin of Luning,Miyi and Caoke chicken breeds in south- western Sichuan province,PCR amplification,DNA collection and sequencing were used to obtain the partial sequence information of mitochondrial DNA （mtDNA） D-loop region from 30 samples of each chicken breed, then DNAMAN, MEGAS,DnaSP,Network were employed to analyze the sequences. The sequence information of 589 bp fragment of mtDNA was obtained from 90 samples, and a Haplotype network and a hierarchical cluster tree of breeds were established. Sequence analysis revealed little genetic differentiation among Luning,Miyi and Caoke chicken populations. Those maternal origins were presumed to be from Callus gallus gallus species in Indochina peninsula,Thailand and Sumatra.

关 键 词：遗传多样性 母系亲缘关系 mtDNA D—loop区 地方鸡品种 

分 类 号：S831.2[农业科学—畜牧学]