卵巢浆液性腺癌中WWOX和PTEN表达及临床病理意义  

Expression and clinicopathologic significance of WWOX and PTEN in ovarian serous adenocarcinoma

作　　者：张军[1] 李月红[2] 崔爱荣[2] 王恒树[2] 

机构地区：[1]河北医科大学第四医院妇科,石家庄050011 [2]河北医科大学第二医院病理科,石家庄050000

出　　处：《临床与实验病理学杂志》2013年第9期976-981,共6页Chinese Journal of Clinical and Experimental Pathology

基　　金：河北省自然科学基金(C2009001229)

摘　　要：目的探讨卵巢浆液性腺癌中WWOX和PTEN的表达及与临床病理特征的关系。方法应用RT-PCR、Western blot、FCM和免疫组化EliVision两步法分别检测卵巢囊腺瘤、交界性囊腺瘤和浆液性腺癌中WWOX和PTEN蛋白及mRNA的表达。结果 RT-PCR:卵巢浆液性腺癌中WWOX和PTEN mRNA阳性率分别为46.7%和51.1%,相对表达量分别为0.22±0.06和0.18±0.08,均显著低于其余各组(P<0.05);Western blot:卵巢浆液性腺癌中WWOX和PTEN蛋白阳性率分别为44.4%和53.3%,相对表达量分别为0.11±0.04和0.34±0.12;FCM:卵巢浆液性腺癌中WWOX和PTEN蛋白FI值分别为0.930±0.020和0.908±0.023;免疫表型:高、低级别卵巢浆液性腺癌中WWOX蛋白阳性率为28.6%和60.0%,PTEN蛋白阳性率为33.3%和62.9%,均明显低于其余各组(P<0.05),高级别浆液性腺癌中WWOX和PTEN阳性率明显低于低级别(P<0.05)。结论卵巢浆液性腺癌中WWOX和PTEN mRNA及蛋白表达明显降低,提示WWOX和PTEN在卵巢浆液性腺癌发生中起一定作用,二者在高、低级别浆液性腺癌中的表达存在差异,进一步提示不同级别的浆液性腺癌具有不同的发生机制。Purpose To investigate the expression of WWOX and PTEN in ovarian serous adenoearcinoma and relationship with clini- eopathologie parameters. Methods WWOX and PTEN protein level were evaluated with EliVision immunohistochemical staining, Western blot and FCM analysis. WWOX and PTEN mRNA level were measured by reverse-transcript polymerase chain reaction （ RT- PC R）. Results The rates of mRNA expression for WWOX and PTEN were 46.7% and 51.1% , the relative quantities were 0. 22 _＋ 0. 06 and 0.18 -＋0.08 in ovarian serous adenocarcinoma, and they were all significant lower than the other goups （P 〈 O. 05 ）. The rates of protein expression for WWOX and PYEN in ovarian serous adenocarcinoma were 44.4% and 53.3% , the relative quantities were 0. 11 -＋0. 04 and 0. 34 -＋ 0. 12 by Western blot. The results of FCM showed that the fluorescence index （FI） for WWOX and PTEN in ovarian serous adenocarcinoma were 0. 930 -＋ 0. 020 and 0. 908 ＋ 0. 023. The IHC results showed that the rates of positive stainning in high and low grade ovarian serous adenocarcinoma were 28.6% and 60. 0% for WWOX, 33.3% and 62. 9% for PTEN re- spectively. Statistically, WWOX and PTEN protein level in high and low grade serous adenoearcinoma were all significant lower than the other groups （ P 〈 0.05 ）. Furthermore, they were obviously lower in high grade than low grade （ P 〈 0. 05 ）. Conclusion The re- sults indicated that the downregulation or loss of WWOX and PTEN expression may contribute to oncogenesis of ovarian serous adeno- carcinoma. Differences in expression of WWOX and PTEN between high and low grade serous adenocarcinoma further suggested that high grade serous adenocarcinoma may have other carcinogenic pathways.

关 键 词：卵巢肿瘤 高级别浆液性腺癌 WWOX PTEN 

分 类 号：R737.31[医药卫生—肿瘤]