低磷胁迫下不同基因型玉米内源激素的动态变化  

Dynamic Change of Endogenous Hormones in Different Maize Genotypes under Low-Phosphorus Stress

作　　者：陈洁[1,2] 张永中[2] 张谦[3] 沈亚欧[2] 高世斌[2] 唐海涛[1] 何文铸[1] 谭君[1] 张彪[1] 潘光堂[2] 

机构地区：[1]四川省农业科学院作物研究所/国家玉米改良分中心,成都610066 [2]四川农业大学玉米研究所/农业部西南玉米生物学与遗传育种重点实验室,成都611130 [3]四川省农业科学院农产品加工研究所,成都610066

出　　处：《玉米科学》2013年第5期6-12,共7页Journal of Maize Sciences

基　　金：国家自然科学基金项目(30971796;30900901);国家科技支撑计划(2011BAD35B01);国家"863"计划(2011AA10A103-2)

摘　　要：用高效液相色谱法测定在低磷胁迫下不同基因型玉米生育进程中叶片和根系内源激素含量的动态变化。结果表明,不同基因型玉米叶片和根系内源激素含量除ABA含量为磷低效自交系>磷高效自交系>F1外,ZT、GA3和IAA含量均为F1>磷高效自交系>磷低效自交系。低磷胁迫下,除ZT含量较正常供磷水平减少外,GA3、IAA和ABA含量均较正常供磷水平增加。ZT和ABA的变幅表现为磷低效自交系大于磷高效自交系和F1,GA3和IAA的变幅表现为磷高效自交系和F1大于磷低效自交系。此外,低磷胁迫下磷高效自交系与磷低效自交系间同一内源激素最值出现时期不同。说明低磷胁迫下不同磷利用效率自交系内源激素表现出规律性变化,因此可将内源激素作为鉴定和选育玉米磷高效基因型的参考指标。Different maize genotypes were selected to assay the dynamic change of endogenous hormone in leaves and roots in the main development process under low-phosphorus stress with high performance liquid chromatography.The results showed that,all of the hormones except ABA were observed more abundant in the high P-efficient lines and F1 than in the low P-efficient lines.The contents of GA3,IAA and ABA in leaves and roots increased significantly under the stress of phosphorus starvation in all of the period,but ZT content decreased after suffering the low-P stress.The change ranges of GA3 and IAA were greater in the high P-efficient lines and F1 than in the low P-efficient lines,whereas it was the opposite case for ZT and ABA contents.In addition,the maximum and minimum emerging period of endogenous hormones content were also different between the high P-efficient inbred lines and the low P-efficient ones when they encountered the stress of phosphorus starvation.The regular changes of endogenous hormone were detected among the different P-availability lines response to the low-P stress,so the endogenous hormone can be considered as an important reference for identifying and breeding high p-efficiency genotype maize.

关 键 词：玉米 低磷胁迫 内源激素 动态变化 

分 类 号：S513.033[农业科学—作物学]