3-乙酰基-11-羰基-β-乳香酸在大鼠体内的代谢途径研究  

Analysis on in vivo metabolic pathways of 3-acetyl-11-keto-β-boswellic acid in rats

作　　者：蔡悠悠[1,2] 夏媛媛[2] 谷元[2] 刘昌孝[2] 司端运[2] 

机构地区：[1]天津中医药大学,天津300193 [2]天津药物研究院释药技术与药代动力学国家重点实验室,天津300193

出　　处：《中草药》2013年第17期2427-2432,共6页Chinese Traditional and Herbal Drugs

基　　金：国家科技重大专项(2012ZX09304002);973项目(2010CB933900)

摘　　要：目的研究3-乙酰基-11-羰基-β-乳香酸(AKBA)在大鼠体内的代谢途径。方法大鼠ig AKBA60 mg/kg后,收集胆汁、尿液及粪便,应用高效液相色谱-四级杆-离子阱质谱(HPLC-QTRAP-MS)技术分析AKBA在大鼠体内的代谢途径。结果共检测到除原形药物外的15个代谢产物,提示AKBA在大鼠体内的主要代谢途径为去乙酰化、单羟基化、脱氢化及葡萄糖醛酸结合物。结论 AKBA在大鼠体内经历了广泛的I相、II相代谢,产生的代谢产物主要通过粪便及胆汁排泄;利用QTRAP技术的自动切换扫描(IDA)软件及增强型产物离子(EPI)扫描功能,能够快速得到高质量的MS2谱图,为AKBA代谢产物的鉴定提供了有效的检测手段。Objective To study the in vivo metabolic pathways of 3-acetyl-11-keto-β-boswellic acid （AKBA） in rats. Methods An HPLC-QTRAP-MS method was applied to identifying the metabolites of AKBA in bile, urine, and feces after ig administration of AKBA 60 mg/kg to rats. Results A total of 15 metabolites were found in rats. The major metabolic pathways of AKBA were deacetylation, mono-hydroxylation, dehydrogenation, and glucuronide conjugates. Conclusion AKBA undergoes the extensive phases I and II metabolism in rats; The metabolites produced mainly excrete through feces and bile; The information-dependent acquisition （IDA） and enhanced product ion （EPI） scan were used on a linear ion trap mass spectrometer to get high quality MS2 picture and provide an effective method for identifying the metabolites of AKBA.

关 键 词：3-乙酰基-11-羰基-β-乳香酸 增强型产物离子扫描 代谢途径 代谢产物 高效液相色谱-四级杆-离子阱质谱 

分 类 号：R969.1[医药卫生—药理学]