生物素-亲和素放大系统荧光免疫分析检测太空杯中的双酚A  

Determination of Bisphenol A in Space Bottle Using Fluorescence Immunoassay with Biotin-avidin Amplification System

作　　者：薛维丽[1] 王新懿[1] 董雯[1] 王术皓[1] 

机构地区：[1]聊城大学化学化工学院,山东聊城252059

出　　处：《河北师范大学学报（自然科学版）》2013年第5期495-499,共5页Journal of Hebei Normal University：Natural Science

基　　金：国家自然科学基金(20877037)

摘　　要：通过简单的全合成将生物素共价结合到抗双酚A抗体上形成生物素化的抗双酚A抗体复合物，结合荧光素标记的亲和素作为荧光探针，建立了测定双酚A的荧光免疫分析新方法．在优化的实验条件下，测定双酚A的线性范围为0．1～10000ng／mL，检出限为0．05ng／mL．将其用于太空杯中双酚A的测定，得到的样品回收率和相对标准偏差分别为91．8％～110．0％和2．2％～5．3％．实验结果表明该方法简单、稳定、可靠．A new fluorescence immunoassay method based on the bioton-avidin amplification system was developed for determination of bisphenol A（BPA）. The complex of anti-BPA antibody and biotin was prepared by a simple procedure,and the fluoresceinisothiocyanate-labelied avidin（FITC-avidin） was regarded as a fluorescence probe in this system. Under the optimal conditions,the linear range for determination of BPA is 0.1-10 000 ng/mL with the detection limit of 0.05 ng/mL. The proposed method was applied to the determination of BPA in space bottle,with the recoveries of 91.8%-110.0 % and the relative standard deviations（RSD） of 2.2 %-5.3%. The experimental results indicated that this method is simple,stable, reliable.

关 键 词：双酚A 生物素-亲和素 荧光免疫分析 太空杯 

分 类 号：O657.3[理学—分析化学]