第四代HIV诊断试剂检测HIV抗体假阳性原因分析  

Causes of false-positive results in p24 Ag /anti-HIV combined assay

作　　者：顾春瑜[1] 刘英丽[1] 王海滨[1] 孙婷婷[1] 何丽婧[1] 

机构地区：[1]解放军总医院第一附属医院检验科,北京100048

出　　处：《武警医学》2013年第9期758-760,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的分析第四代HIV诊断试剂检测HIV抗体假阳性的原因,减少假阳性检出率。方法选取119例HIV四代试剂检测初筛阳性的标本,送HIV确认实验室确认HIV抗体结果,分析比对两者的结果,探讨出现假阳性的原因。结果119例HIV抗体初筛阳性的标本,送至确认实验室回报结果 40例HIV抗体阳性,28例HIV抗体不确定,51例HIV抗体阴性。随着S/CO值的增高,初筛结果与确证结果阳性符合率也随之升高。结论 HIV四代检测试剂假阳性率较高,这与试剂同时检测HIV抗体和P24抗原有关。当1<S/CO≤10时,实验室检测人员应依据实验检测结果,结合受检者相应病史和个体状况进行综合分析。Objective To stud）, the causes of false positive results in the fourth generation p24 Ag/anti - HIV combined assay and cut down the false positive results. Methods We picked 119 samples which were reactive in HIV - Ab screening test with the fourth generation HIV assay, then confirmed these samples with West - blot assay and compared them. Results In 119 reactive samples, 40 samples were HIV - 1 positive, 28 samples were HIV - Ab indeterminate, and 51 samples were HIV - Ab negative. The higher the S/CO index, the closer the positive results of screening test related to one of confirmatory test. Conclusions The rate of false positive is high with the fourth generation HIV assay, which is relative to the combined detection of anti - HIV - P24. When 1 〈 S/CO ≤ 10, we should consider comprehensive factors such as history of disease, the clinical symptoms, etc.

关 键 词：HIV抗体 HIV-1P24抗原 HIV四代试剂 假阳性反应 

分 类 号：R446.6[医药卫生—诊断学]