免疫抑制治疗对受体上皮细胞灌注后大鼠气管异位移植再上皮化影响  

EFFECT OF IMMUNOSUPPRESSIVE THERAPY ON RE-EPITHELIZATION AFTER RAT HETEROTOPIC TRACHEAL GRAFT WITH RECIPIENT EPITHELIAL CELLS PERFUSION

作　　者：张轶[1] 胡学飞[1] 陈昶[1] 

机构地区：[1]同济大学附属上海市肺科医院胸外科,上海200433

出　　处：《青岛大学医学院学报》2013年第6期487-491,共5页Acta Academiae Medicinae Qingdao Universitatis

基　　金：上海市科委资助项目(114119a9400)

摘　　要：目的探讨免疫抑制治疗对受体源性的再上皮化过程的影响,以及免疫抑制治疗的最佳方案。方法将60只大鼠随机分为6组,每组10只,建立大鼠气管异位移植模型。对照组1:大鼠气管内注入生理盐水;对照组2:大鼠气管内注入受体上皮细胞悬液;实验组1:大鼠气管内注入受体上皮细胞悬液,移植后每日肌肉注射他克莫司(FK506)0.5mg/kg,共3d;实验组2:大鼠气管内灌注受体上皮细胞悬液,移植后每日肌肉注射FK506 0.5mg/kg,共10d;实验组3:气管内灌注受体上皮细胞悬液,移植后每日肌肉注射FK506 1.0mg/kg,共3d;实验组4:气管内灌注受体上皮细胞悬液,移植后每日肌肉注射FK506 1.0mg/kg,共10d。移植后第28天,处死大鼠,取气管制备标本,分别行苏木精-伊红(HE)染色、淋巴细胞浸润计数,上皮细胞CK14、CK18和CFTR分化鉴定,以及透射电镜观察,观察各组大鼠气管异位移植再上皮化的情况。结果对照组1未见任何上皮细胞再生,管腔出现严重狭窄、闭塞;对照组2也未实现再上皮化,但管腔狭窄、闭塞程度稍好于对照组1;实验组1和实验组2气管内壁仅局部有上皮细胞再生,且细胞尚未分化成熟;实验组3和实验组4受体源性再上皮化取得成功,气管内覆盖了完整连续的成熟的纤毛柱状上皮细胞,并具有腺体分泌功能、屏障防御功能。结论气管内注入受体上皮细胞后再实施异位移植,在一定的条件下可实现受体源性的完全再上皮化。Objective To study the influence of immunosuppressive therapy on re-epithelization from the recipient, and the preferred plan of the therapy. Methods Sixty rats were evenly randomized to six groups, and a heterotopic subcutaneous tracheal transplantation model in rat was created. Control group 1： intratracheal injection of normal saline; Control group 2： intra- tracheal injection of recipient epithelial cells suspension （RECS） ： Experimental group 1 ： intratracheal iniection of RPCS, and, after transplantation, I.M. injection of Tacrolimus （0.5 mg · kg ^-1 · d^-1）, daily for 3 days; Experimental group 2： intratracheal injec- tion o5 RPCS, and with LM. Injection 05 Tacr01imus （0.5 mg· kg ^-1 · d^-1） for 10 days after transplantation; intramuscular injec- tion of Tacrolimus （0.5 mg· kg ^-1 · d^-1） for ten days; Experimental group 3： intratracheal injection of RPCS, and I.M. injection of Tacrolimus （1.0 mg· kg ^-1 · d^-1） for 3 days after transplantation; Experimental group 4： intratracheal injection of RPCS, and I.M. injection of Tacrolimus （1.0 mg · kg ^-1 · d^-1） for 10 days after transplantation. The rats were killed on day 28 after trans- plantation, the implanted tracheas were harvested. The histological and ultrastruetural changes, infiltrated lymphocytes and differ- entiation of CK14, CK18, and CFTR were evaluated. Results In the control group 1, no any epithelial cell regeneration was ob- served, and severely narrowed and occluded lumen appeared; in the control group 2, no epithelization was observed as well, but the condition of the lumen was a slightly better than that in the former group; in experimental groups 1 and 2, only local epithelial re- generation, hut immature, could be seen; in experimental groups 3 and 4, the epithelization was succeeded. The inner surface of trachea was covered by completely well-developed epithelial ceils with gland secretion function and barrier function. Conclusion A heterotopic tracheal grafting after intratracheal injectio

关 键 词：气管 移植 上皮细胞 免疫抑制剂 

分 类 号：R655.3[医药卫生—外科学]