石墨炉原子吸收光谱法测定昆明鼠器官铂含量  

Determination of Platinum in Tissues of KM Mice by Flameless Atomic Absorption Spectrophotometry

作　　者：耿旭[1] 黄静[2] 张贵荣[3] 

机构地区：[1]河南大学医学院,河南开封475004 [2]华东师范大学生命科学学院,上海200062 [3]华东师范大学理工学院化学系,上海200062

出　　处：《河南师范大学学报（自然科学版）》2013年第5期120-122,共3页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(31170920)

摘　　要：研究高分子顺铂(PGA-Asp-CDDP)腹腔给药昆明鼠,1h后取出脏器检测其铂含量.石墨炉原子吸收光谱测定获得的铂浓度标准曲线,其相关系数为0.997 5,特征浓度为2.4μg·L-1;并考察了消解液用量和消解温度对测定铂浓度的影响;回收率实验表明该消解方法在测定铂浓度上具有可行性和可靠性.在此基础上,昆明鼠的心脏、肝脏、脾脏、肺和肾脏的铂含量检测结果分别为(5.46±0.89)、(28.65±13.45)、(45.30±4.07)、(16.90±4.74)和(76.31±2.59)μg·g-1.The concentrations of Platinum in the heart,liver,spleen,lung,and kidney removed from Kunming mice 1h after they had received intraperitoneal poly（γ-glutamic acid）asp-cisplatin complex（PGA-Asp-CDDP）was determinated.The calibration curve of platinum concentration was obtained by flameless atomic absorption spectrophotometry,the correlation coefficient and the characteristic concentration of the curve were 0.9975and 2.4μg/L.In the study,the effects of experimental conditions,including the amount of acid and digestion temperature on the determination of platinum of PGA-Asp-CDDP were investigated detailedy.Recovery experiment showed that the solubilization method of PGA-Asp-CDDP and tissue was feasible and reliable.On this basis,the Kunming mouse＇s heart,liver,spleen,lungs and kidneys to be detected,and platinum contents of tissues were（5.46±0.89）,（28.65±13.45）,（45.30±4.07）,（16.90±4.74）and（76.31±2.59）μg/g,respectively.

关 键 词：原子吸收光谱 顺铂 高分子药物 铂(Ⅳ) 器官分布 

分 类 号：O657.3[理学—分析化学]