免疫印迹法带型作为判定HIV-1新发感染的研究  

Study on the role of western blot band profile for the detection of recent HIV infection

作　　者：王继宝[1] 张娜[2] 于海英[3] 李艳玲[1] 段星[1] 颜浩[3] 杨锦[1] 段松[1] 蒋岩[3] 

机构地区：[1]云南省德宏州疾病预防控制中心,芒市678400 [2]江西省疾病预防控制中心 [3]中国疾病预防控制中心性病艾滋病预防控制中心参比实验室

出　　处：《中华流行病学杂志》2013年第10期998-1002,共5页Chinese Journal of Epidemiology

摘　　要：目的从HIV确证的免疫印迹法（wB）带型中获得新发感染相关信息。方法收集云南省德宏州2011年经WB确证为HIV抗体阳性样本1466份，分别用BED捕获酶免疫法（BED—CEIA）和限制性抗原亲和力酶联法（LAg．AvidityEIA）检测新发感染者，分析比较WB检测中不同条带特征对新发感染判定的意义。结果BED．CEIA或LAg．AvidityEIA检出的新发感染者，其WB的p17、p24、p31、gp41、p51、p55、p66、gpl20和gpl60条带反应强度与长期感染的差异有统计学意义（P〈0．05），均低于长期感染者相应的条带反应强度。结论WB条带中的p31呈阴性反应、WB条带反应强度总分不高于13分、env区条带反应强度不高于5分或pol区条带反应强度不高于4分时应高度怀疑其为新发感染。Objective To obtain information on HIV disease progression through western blot （WB） band profile. Methods 1466 available serum samples of newly reported HIV/AIDS cases, confirmed by WB from Dehong prefecture of Yunnan province in 2011, were tested, using the BED HIV Incidence Capture Enzyme Immunoassay （BED-CEIA） and Limiting Antigen Avidity Enzyme Immunoassay （LAg-Avidity EIA） , respectively. Differences of WB band profiled between recent infections and established infections were then compared. Results Infections defined as ＇recent＇ showed lower mean antibody intensities to p17, p24, p31, gp41, p51, p55, p66, gpl20 and gpl60 in WB when compared to those established infections with statistical difference （P〈0.05）. Conclusion These WB band profiles which were considered as ＇recent＇ infections would include factors as ： lack of antibody reactivity to gp41 antigen, with intensity scores of all bands lower than 13, and those intensity scores of antibody to env protein lower than 5 or to pol protein lower than 4.

关 键 词：HIV新发感染 免疫印迹法带型 

分 类 号：R446.6[医药卫生—诊断学]