百日咳实验室诊断方法的应用分析与比较  

Comparative studies on different methods for laboratory diagnosis of pertussis

作　　者：王增国[1] 杨杨[1] 刘莹[1] 刘小乖[2] 习艳丽[1] 雷玲霞[2] 李亚绒[2] 

机构地区：[1]西安市疾病预防控制中心,710054 [2]西安市儿童医院

出　　处：《中华流行病学杂志》2013年第10期1010-1012,共3页Chinese Journal of Epidemiology

基　　金：陕西省科技计划项目（2012k16-05-08）;西安市卫生局科技项目（J2011049）

摘　　要：目的利用〈1岁临床百日咳疑似病例实验室检测结果，分析比较不同检测方法。方法采集2011年12月至2012年12月西安市儿童医院〈1岁百日咳临床疑似病例的鼻咽拭子及血清标本，采用分离培养、IS481PCR及百日咳毒素（PT）IgG定量酶联免疫吸附试验（PT-IgG）检测百日咳鲍特菌感染，并应用SPSS16．0软件分析结果。结果148例临床疑似病例中实验室诊断病例100例，其中3例百日咳鲍特菌分离培养阳性，88例IS481PCR阳性，34例PT-IgG阳性，IS481PCR和PT-IgG均阳性22例。IS481PCR及PT-IgG检测结果与患儿发病天数的差异有统计学意义（P〈0．01）。结论百日咳鲍特菌分离培养方法敏感度较低，而采用IS481PCR和PT-IgG两种方法还应结合发病天数加以判断。Objective To confirm the clinically suspected pertussis cases （ 〈 1 years old） through laboratory methods. Methods From December, 2011 to December, 2012, patients with clinically suspected pertussis from Xi＇ an Children＇ s Hospital were sampled, with their nasopharyngeal swabs collected, blood samples cultured and pertussis toxin IgG detected by PCR. Results were analyzed,using SPSS 16.0 software. Results 100 out of the 148 cases were laboratorially confirmed. 3,88 and 34 cases were positive, through culture, PCR or pertussis toxin IgG respectively. 22 cases were both PCR and pertussis toxin IgG positive. There were significant differences between the results of IS481 PCR, days from the onset of symptoms （P〈0.01） and results of PT-IgG with the days from onset of symptoms （P〈0.01）. Conclusion Since the sensitivity of culture on pertussis was low, diagnosis on the disease should be linked to the results from PCR, PT-IgG and the days from onset of symptoms.

关 键 词：百日咳 酶联免疫吸附试验 聚合酶链反应 

分 类 号：R446.6[医药卫生—诊断学]