稻米胶稠度基因位点的标记和分析  

Molecular Mapping QTLs for Gel Consistency in Rice (Oryza sativa L.)

作　　者：黄祖六[1] 谭学林[2] 徐辰武[1] Vanavichit A. 

机构地区：[1]扬州大学农学院数量遗传研究室,江苏扬州225009 [2]云南农业大学水稻研究所 [3]Agriculturalengineeringcenter

出　　处：《中国农业科学》2000年第6期1-5,共5页Scientia Agricultura Sinica

基　　金：洛克菲勒基金项目;国家自然科学基金资助项目 !( 3990 0 0 80 )

摘　　要：以硬胶稠度 (GC)的 CT9993和中等胶稠度的 KDML1 0 5水稻品系杂交产生的 1 52个重组近交系 (RIL)为材料 ,用 RFLP、AFLP和微卫星 (SSLP)标记构建了 3张分子标记连锁图 ,以此对控制稻米 GC的数量性状位点 (QTL)进行了分析。结果表明 ,稻米 GC主要受位于第 3染色体的两个连锁位点控制 ,它们分别位于 R2 1 70左、右两侧 8.8c M和 1 7c M处。上述两个位点都以大于 1 6.0的 LOD值检测到。控制 GC的微效 QTL的数目则因连锁图的不同而异 ,约为A population of 152 recombinant inbred lines (RILs) derived from a cross between CT9993, a japonica rice with hard gel consistency (GC) and Khoa Dawk Mali105 (KDML 105 ), a Thai famous rice with medium GC, was used for GC quantitative trait loci (QTLs) analysis. Three linkage maps were constructed with RFLP , AFLP and microsatellite (SSLP) markers. The first one consists of 83 RFLP markers with 17.53 cM of an average distance between markers. The second one consists of 83 RFLP and 69 AFLP markers with 13.22 cM average distance between markers and the third one consists of 83 RFLP, 69 AFLP and 15 SSLP markers, and has an average distance of 12.98 cM between markers. Based on these three maps, QTLs conferring GC were analyzed. The results show that GC is mainly controlled by two linked loci,which are located at the two flanks of RFLP marker R2170 on chromosome 3 .The distance between the two linked QTLs is 25 cM ,which were determined by a LOD scores larger than 16.0. The number of minor QTLs controlling GC is varying from 4~9 in the three different linkage maps.

关 键 词：稻米品质 胶稠度 数量性状 位点 分子标记连销图 

分 类 号：S511.032[农业科学—作物学]