磁共振波谱成像观察大鼠大脑中动脉闭塞的针刺治疗效果  

Effect of Acupuncture on Middle Cerebral Artery Occlusion in Rats Observed by Magnetic Resonance Spectroscopy

作　　者：李欢欢[1] 黄丙仓[1,2] 许晓岚[2] 武刚[1] 

机构地区：[1]复旦大学附属中山医院青浦分院放射科,上海201700 [2]上海市浦东新区公利医院医学影像科,上海200135

出　　处：《中国临床医学》2013年第3期379-382,共4页Chinese Journal of Clinical Medicine

基　　金：上海市科技发展基金(编号:114119a9700);上海市浦东新区科技发展基金(编号:PKJ2011-Y10)

摘　　要：目的:采用基于磁共振成像(magnetic resonance imaging,MRI)的磁共振波谱成像(magnetic resonance spectroscopy,MRS)观察针刺对大鼠大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)的治疗效果。方法:将60只雄性Sprague-Daw-ley(SD)大鼠随机分为针刺组(n=27)、模型组(n=27)、假手术组(n=6),针刺组和模型组再按MRI检查时段分为3个亚组(每个亚组9只):24 h亚组、3 d亚组和7 d亚组。采用改良线栓法构建MCAO模型,在造模后行MRI、氢质子磁共振波谱成像(1H-MRS)检测,并对图像进行处理和定量分析。结果:MCAO模型7 d亚组的大鼠栓塞区T2WI高信号范围较24 h亚组和3d亚组有缩小趋势,假手术组T2WI未见明显异常信号;1H-MRS检测结果表明,针刺3 d亚组和针刺7 d亚组N-乙酰天门冬氨酸(N-acetylaspartate,NAA)较模型组明显升高(31.43±2.88)比(25.90±3.48,60.84±2.76比54.31±1.40;P均<0.05),乳酸(lactate,Lac)明显降低(58.82±3.78比65.15±3.58,50.22±4.33比55.46±4.06;P均<0.05),而胆碱复合物(choline,Cho)、肌酸(creatine,Cr)差异无统计学意义(P>0.05)。结论:1H-MRS检查能够客观评价针刺处理对MCAO大鼠梗死灶的干预作用,是研究脑梗死针刺治疗的可靠影像学方法。Objective：To observe the treatment effect of acupuncture on middle cerebral artery occlusion（MCAO） in rats through magnetic resonance spectroscopy（MRS） based on magnetic resonance imaging（MRI）.Methods：A total of 60 male Sprague-Dawley rats were randomly divided into acupuncture group（27 cases）,model group（27 cases） and sham operation group（6 cases）.The acupuncture group and model group were further randomly divided into three subgroups（9 cases in each subgroup）： 24 h subgroup,3 d subgroup and 7 d subgroup.Rat MCAO model was constructed by modified inserting thread method.MRI and 1H-MRS were examined at the corresponding time points,and the images were further processed and quantitatively analyzed.Results： After MCAO model construction,the lesion areas with high T2WI signal intensity in 7 d subgroup had a narrowing tendency compared with 24 h subgroup and 3 d subgroup.No obvious abnormal signals were observed in the sham operation group.1H-MRS assay results showed that there were significant increases of N-acetyl aspartate（NAA） in 3 d subgroup and 7 d subgroup of acupuncture group compared with those of model group（31.43±2.88 vs.25.90±3.48,60.84±2.76 vs.54.31±1.40,both P0.05）,but lactate（Lac） was decreased（58.82±3.78 vs.65.15±3.58,50.22±4.33 vs.55.46±4.06,both P0.05）.However,no significant differences were found in choline（Cho） and creatine（Cr）（P0.05）.Conclusions： 1H-MRS can objectively assess the intervention effect of acupuncture treatment on the lesions of rats with MCAO,and it is a reliable imaging method for researches on acupuncture treatment in cerebral ischemic stroke.

关 键 词：磁共振波谱成像 缺血性脑卒中 针刺 大脑中动脉闭塞模型 动物实验 

分 类 号：R814.49[医药卫生—影像医学与核医学]