水溶性荧光素荧光探针用于草鱼中孔雀石绿残留量的测定  

Determination of Residual Amounts of Malachite Green in Grass Carp with Fluorescence Probe of Water Soluble Fluorescein

作　　者：梁宇[1] 黄世江[2] 程定玺[3] 王超海[1] 

机构地区：[1]新乡学院化学与化工学院,新乡453003 [2]河南科技学院新科学院,新乡453003 [3]河南师范大学化学化工学院,新乡453007

出　　处：《理化检验（化学分册）》2013年第9期1034-1037,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：河南省重点科技攻关项目(102102210043)

摘　　要：水于水溶性荧光素(SF)与孔雀石绿(MG)的荧光猝灭作用,提出了用荧光素作为荧光探针测定了草鱼中孔雀石绿残留量的方法。试验发现.在pH 7.0的B-R缓冲浓液中.孔雀石绿的质量浓度在80μg·L^(-1)范围内与荧光猝灭强度呈线性关系,方法的检出限(3S/N)为0.01μg·L^(-1)。方法用于草鱼样品中孔雀石绿的测定,用标准加入法做回收试验,测得回收率在68.4%~83.3%之间,测定值的相对标准偏差(n=6)在3.4%~5.3%之间。Based on the fluorescence quenching action of malachite green （MG） on water soluble fluorescein （SF）, a method for determination of residual amount of MG in grass carp using SF as fluorescence probe was proposed, h was found that in a B-R buffer solution, linear relationship between the magnitude of fluorescence quenching and mass concentration of MG was kept in the range within 80 μg ·L-1 , with detection limit （3S/N） 0. 01 μg ·L -1. Recovery of the method was tested by standard addition method, values of recovery found were in the range of 68. 4%-83.3% with RSD （n=6） in the range of 3.4%-5.3%.

关 键 词：荧光探针 水溶性荧光素 孔雀石绿 

分 类 号：O657.31[理学—分析化学]