DNA-不对称席夫碱及其配合物的合成表征及其荧光性质的研究  

Syntheses, Characterization and Luminescent Properties of DNA-asymmetric Schiff Base Compounds

作　　者：郭华[1] 时茜[1] 

机构地区：[1]温州大学化学与材料工程学院,浙江温州325035

出　　处：《辽宁化工》2013年第9期1023-1025,共3页Liaoning Chemical Industry

基　　金：国家自然科学基金;项目号:KZ0911008

摘　　要：设计合成两条端基为胺基修饰的互补的DNA单链D1和D2分别与3-氯水杨醛和5-氯水杨醛反应,成功得到2种DNA-席夫碱单链化合物S1、S2,利用DNA-席夫碱单链间的配对作用,合成得到1种DNA-不对称席夫碱化合物L1。相关席夫碱化合物分别与Cd(II)、Cu(II)、自组装,得到2种不对称的席夫碱配合物Cd2(L1)(en)2Cl2(1)与Cu2(L1)(en)2Cl2(2)。利用紫外-可见吸收光谱对反应进程进行监测,通过凝胶电泳技术对DNA-席夫碱单链化合物、DNA-不对称席夫碱化合物进行分离和表征,并对所得席夫碱以及相应配合物的荧光性质进行了研究。Two DNA-schiff base single strand compounds （S1, S2） were synthesized by reaction of two complementary base pairing amino-modified DNA single strands with 3-chloro-salicylaldehyde and 5-chloro-salicylaldehyde, respectively. Then one asymmetric DNA-schiffbase compounds L1 was synthesized by using the base pairing function of the DNA-schiff base single strand compounds. Two schiff base complexes Cd2（L1）（en）2Cl2（1）,Cu2（L1）（en）2 Cl2（2） were synthesized by Schiff base compounds self-assembling with Cd （II）, Cu （II） respectively.The reaction processes were monitored by ultraviolet-visible absorption spectrum. The DNA-schiff base single strand compounds and the DNA-schiff compounds were characterized by polyacrylamide gel electrophoresis,and their fluorescent properties were also studied.

关 键 词：DNA-不对称席夫碱配合物 紫外-可见吸收光谱 凝胶电泳 荧光性质 

分 类 号：TQ031[化学工程]