高强度聚焦超声增效及超声显像技术研究  

Study on High Intensity Focused Ultrasound Synergism and Ultrasound Imaging Technology in Vitro

作　　者：何坤燕[1] 郝兰[1] 冉海涛[1] 王志刚[1] 郑元义[1] 

机构地区：[1]重庆医科大学超声影像学研究所,重庆医科大学附属第二医院超声科,重庆400010

出　　处：《压电与声光》2013年第5期631-635,共5页Piezoelectrics & Acoustooptics

基　　金：国家自然科学基金资助项目(No.81130025;No.81071157)

摘　　要：首先采用超声乳化离心法制备载液态氟碳全氟己烷(PFH)富勒烯纳米球(PFH-C60),然后应用高强度聚焦超声(HIFU)对各组样品进行辐照后行体外超声显像,再另取新鲜离体牛肝,分别局部注射生理盐水、C60溶液及PFH-C60溶液后,给予相同参数的HIFU辐照,观察各组辐照区域视频灰度变化及肿瘤凝固性坏死情况。实验结果发现,PFH-C60可增强HIFU消融效果及体外超声显像;PFH-C60体外超声显像呈无回声,行一定参数HIFU辐照后超声信号明显增强;牛肝注射PFH-C60后行HIFU辐照,较NS组及C60组辐照区灰度变化面积和变化值有显著差异(误差概率P<0.01),且凝固性坏死体积明显增大(P<0.01)。实验研究表明,载液态氟碳富勒烯纳米球具备超声成像与增效HIFU的功能。The fullerene nanocapsules of liquid perfluoroearbon （PFH-C60） were prepared by using the phacoe- mulsification extraction method first. Then each group of sample was observed the ultrasound imaging after ablation of high intensity focused ultrasound in vitro. In addition, the degassed bovine liver in vitro was directly injected into normal saline,C60 solution or PFH-C00 separately before HIFU ablation. After HIFU ablation of same parameters, the gray changes of irradiation areas and coagulation necrosis in liver were observed. The results of experiment found that PFH-C^0 can enhance HIFU ablation and ultrasound imaging in vitro,and the PFH-C60 ultrasound ima- ging in vitro was echoless, but after irradiated by HIFU （certain scope of parameters）, it got an echo enhancement. After proceeding H1FU ablation, compared with group NS and C60 ,group PFH-C60 showed significant differences in gray change values and squares （P^0.01）, also whose volume of coagulation necrosis became larger （P%0.01）. The research indicates that the prepared PFH-C60 nanocapsules have the functions of Ultrasound imaging and HIFU synergist in vitro.

关 键 词：高强度聚焦超声（HIFU） 增效 显像 液态氟碳全氟己烷（PFH） 富勒烯 

分 类 号：R454.3[医药卫生—治疗学]