鲤鱼外周血白细胞TLR5M全长cDNA克隆及序列分析  

Cloning and Sequence Analysis of TLR5M Full-length cDNA in Common Carp Peripheral Blood Leukocytes

作　　者：孙真[1] 贾生美[1] 冯祥汝[1] 陈义龙[1] 翟新新[1] 沈雪飞[1] 张俊辉[1] 王文东[2] 杨振国[1] 卢强[1] 

机构地区：[1]吉林大学人兽共患病研究所,吉林长春130062 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国畜牧兽医》2013年第9期41-45,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30972277)

摘　　要：本试验以鲤鱼TLR5M的EST序列为基础进行5'-RACE试验,获得了其cDNA的全长序列。结果表明,该序列共3182 bp,包含38 bp的5'端非编码区,486 bp的3'端非编码区,1个2658 bp的开放阅读框(ORF),共编码885个氨基酸。序列同源性比对结果表明,该序列与麦瑞加拉鲮鱼TLR5基因同源性高达84.46%。In this paper, we have isolated, sequenced and characterized the full-length cDNA of TLR5M gene from common carp by the methods of rapid amplification of cDNA ends（RACE） on the basis of its EST sequences. The results showed that the TLR5M gene was 3182 bp in length,vthe gene consists of a 38 bp 5＇ untranslated region（UTR）, 486 bp 3＇UTR and a 2658 bp open reading frame（ORF）, encoding a peptide of 885 amino acids. The amino acid sequence of carp TLR5 gene shared 84.46% identity with cirrhinus mrigala.

关 键 词：鲤鱼 TLR5M 克隆 序列分析 

分 类 号：Q78[生物学—分子生物学]