藏绵羊乳腺乳铁蛋白的克隆及序列分析  

Cloning and Sequence Analysis of Lactoferrin Gene in Tibetan Sheep

作　　者：李建波[1,2] 江明锋[1,2] 王永 

机构地区：[1]青藏高原动物遗传资源保护与利用四川省重点实验室,四川成都610041 [2]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《中国畜牧兽医》2013年第9期86-90,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划(2012BAD13B06);四川省科技创新产业链示范工程重大项目(2011NZ0003);中央高校基本科研业务费专项资金项目(11ZYXS24;12ZYXS);四川省2011年度学术技术带头人和留学归国人员项目

摘　　要：本研究以四川若尔盖县藏系绵羊为材料,利用RT-PCR方法扩增并克隆了藏绵羊乳铁蛋白(lactoferrin,LF)序列,运用生物信息学软件对其核苷酸序列和编码蛋白质的结构进行预测和分析。结果表明,克隆的LF基因全长2127 bp,共编码708个氨基酸,N端有19个氨基酸组成的信号肽,该蛋白质等电点为8.4,预测分子质量为77.2 ku;半定量RT-PCR分析结果表明,LF在乳腺、气管、淋巴、肝脏、泪腺和脾脏中都有表达,在肺脏中不表达。本试验克隆了藏绵羊LF cDNA全序列,并对LF和乳铁蛋白肽(lfcin,Lfc)核苷酸和蛋白质进行了分析,为进一步研究其生物学活性奠定基础。RT-PCR method was used to clone the full length of lactoferrin （LF） cDNA sequence in Tibetan sheep at Ruoergai county of Sichuan province. Nucleotide sequence and structure of LF amino acid sequence were deduced by bioinformatics software. The results showed that LF gene was 2127 bp in length and encoded 708 amino acids, including a signal peptide of 19 amino acids at its N terminal. Isoelectric point （pI） of this protein was 8.4, and the molecule mass of the predicted protein was 77.2 ku. Semi-quantitative RT-PCR results indicated that LF gene was expressed in mammary, trachea, lymph, liver, lachrymal and spleen, but not in lung. In conclusion,LF gene in Tibetan sheep had been successfully cloned and the nucleotide and amino acid sequence of LF and Lfc were analysed in this study, it laid a foundation for further research.

关 键 词：藏绵羊 乳铁蛋白基因 乳铁蛋白肽 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]