env基因对J亚群禽白血病病毒体外感染和复制能力的影响  

Effects of env Gene on Subgroup J Avian Leukosis Virus Infection and Replication

作　　者：吴晓婵[1] 李娇 曹伟胜[1] 廖明[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广州畜牧科学研究所,广东广州510545

出　　处：《中国畜牧兽医》2013年第9期143-146,共4页China Animal Husbandry & Veterinary Medicine

基　　金：NSFC-广东联合基金(U0831002);国家肉鸡产业技术体系项目(nycytx-42-G3-03);广东省自然科学基金项目(S2011010001946;10451064201005432)

摘　　要：为探讨env基因对J亚群禽白血病病毒(subgroup J avian leukosis virus,ALV-J)体外感染和复制能力的影响,本研究利用反向遗传方法构建重组病毒,将血管瘤病变型ALV-J HN06株中env元件替换至髓细胞瘤病变型ALV-J NX0101株的相应位置,成功构建了重组质粒pNX-HNenv。重组毒株能在DF-1细胞上稳定增殖,并能被JE9特异性单抗识别,证明获得了具有感染性的重组病毒NX-HNenv株。结果显示,同一亚群内env基因的替换对病毒的体外感染和复制能力无明显影响。In order to rescue the env element-substitution virus, we constructed the recombinant plasmid pNX-HNenv that contained the whole myelomas strain ALV-J NX0101 genome, but with env element of hemangiomas strain ALV-J HN06. The plasmids were transfected into DF-1 cells and the rescued viruses were identified, respectively. The recombinant virus was stable in cell culture during nine passages, and could be identified by ALV-J specific monoclonal antibody JE9 and virus NX-HNenv was rescued. The results showed that the env substitution had no apparent influence on ALV-J virus rescue, virus entry and replication.

关 键 词：J亚群禽白血病病毒 ENV基因 反向遗传技术 复制能力 

分 类 号：Q344.13[生物学—遗传学]