犬瘟热病毒免疫蛋鸡血清抗体与卵黄抗体消长规律的研究  

Dynamics Research of Serum and Egg Yolk Antibody of Laying Hens Immuned by Canine Distemper Virus

作　　者：庄金秋[1,2] 梅建国[2] 姚春阳[1,2] 沈志强[2] 丁壮[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]山东省滨州畜牧兽医研究院,山东滨州256600

出　　处：《中国畜牧兽医》2013年第9期214-217,共4页China Animal Husbandry & Veterinary Medicine

基　　金：山东省优秀中青年科学家科研奖励基金项目(BS2011SW026)

摘　　要：试验旨在了解产蛋鸡对犬瘟热病毒（canine distemper virus,CDV）抗原的免疫反应及其血清抗体和卵黄抗体的消长规律,为制备卵黄抗体提供依据。将CDV接种Vero细胞和DF1细胞进行传代并测定其病毒滴度,选取细胞病变出现早、病毒滴度高的Vero细胞毒作为接种病毒抗原免疫蛋鸡。每10 d免疫蛋鸡1次,第4次免疫后每30 d加强免疫1次。免疫前及免疫后每10 d采血分离血清,每5 d收集鸡蛋提取卵黄抗体,用琼脂扩散法和间接ELISA法测定其抗体水平。结果显示,血清抗体比卵黄抗体的滴度高,两者呈正相关性,卵黄抗体出现较血清抗体迟3~5 d,卵黄抗体在第3次免疫后3~5 d就已达到较高水平,此时即可开始收集鸡蛋制备卵黄抗体。In order to provide the basis for the preparation of egg yolk antibody,the dynamics of the serum antibody and egg yolk antibody in laying hens against canine distemper virus （CDV） antigen was studied,Vero cell and DF1 cell cultures were inoculated with CDV to detect the virus titer. Select the Vero cytotoxic strain with earlier cytopathic and high virus titers as inoculated virus antigen to immunize laying hens. The immune method was to immunize laying hens once every 10 days, for four times successively, then to strengthen the immune every 30 days. Blood sera were separated every 10 days and egg yolk antibodies were collected every 5 days before and after immunization,the antibody levels were determined by agar diffusion and indirect ELISA. The results showed that serum antibody titer was higher than egg yolk antibody, and both were positively correlated,the appearance of egg yolk antibody was later for 3 to 5 days than serum antibody. Egg yolk antibody had reached the highest level in 3 to 5 days after the third immunity, and then you could start to collect eggs to prepare egg yolk antibody.

关 键 词：犬瘟热病毒 血清抗体 卵黄抗体 消长规律 

分 类 号：S852.4[农业科学—基础兽医学]