小麦苗期钾、钠吸收相关性状及其QTL分析  

Potassium and sodium absorption related traits and QTL mapping at the seedling stage of wheat

作　　者：吴春红[1] 梁雪[1,2] 李斯深[3] 李絮花[1] 宫晓平[1] 孔凡美[1] 

机构地区：[1]山东农业大学资源与环境学院,山东泰安271018 [2]山东省菏泽市曹县农业局,山东曹县274400 [3]山东农业大学农学院,山东泰安271018

出　　处：《植物营养与肥料学报》2013年第5期1025-1036,共12页Journal of Plant Nutrition and Fertilizers

基　　金：国家自然科学基金(31201671);山东省自然科学基金(ZR2011CQ033)项目资助

摘　　要：为进一步了解小麦苗期钾、钠吸收的相关性和遗传特征,本试验在温室环境下,以RIL(Recombinant Inbred Lines,RIL)群体为试验材料,采用营养液培养法对不同K+、Na+浓度处理条件下小麦苗期生长及钾、钠的积累、分配及利用等相关的20个性状进行了鉴定,探讨了K+和Na+的关系及其对小麦苗期生长的影响;并对所有相关性状进行了QTL(Quantitative trait loci,QTL)分析。结果表明,缺钾条件下,供应钠能够显著提高小麦体内的Na/K比,但对小麦的生物量没有显著影响,表明钠替代钾的能力较差。而正常钾条件下,供钠可显著促进钾、钠的吸收,提高了Na/K比,但地上部的生物量反而显著下降,表明大量的钾和钠的累积对小麦地上部生物量的形成不利。小麦苗期对钾、钠离子的吸收与累积呈显著正相关。本试验在小麦全部的21条染色体上共检测到与生物量及钾、钠相关性状有关的QTLs 141个。其中的103个QTLs组成了14个重要的QTL簇,有11个QTL簇同时定位了钾、钠相关性状位点。另外,试验还发现了8个在多个养分环境条件下均可检测到的QTL位点—高频表达的QTL位点(RHF-QTLs),其中4个是新发现的位点。这些重要的QTL位点为K+、Na+关系及其遗传控制的深入研究提供了重要参考。Accumulation of K in vacuoles creates necessary osmotic potential for cell extension. Rapid cell extension requires high mobility of osmotic pressure. Na is one of the few other ions which can replace K in this role. To further understand correlation and genetic characteristics of potassium （K） and sodium （Na） absorption and utilization at wheat seedling stage, a hydroponics experiment was conducted with different K and Na treatments in greenhouse using a recombinant inbred lines （RILs）. Variance and correlation analysis were conducted for shoot and root biomass, K and Na accumulation respectively. QTL mapping for twenty related traits including the biomass and K/Na related traits was also conducted. The results show that the accumulations of K and Na in wheat seedlings are closely related to each other. Compared with the K deficiency treatment, the Na/K ratio is significantly increased when supplied with Na, but the biomass of wheat seedlings is not increased, so Na has poor ability to replace K when K is deficient. On the other hand, when K is efficient, the Na＋ treatments can significantly promote K＋ accumulation, but decrease shoot biomass. Therefore, excess amounts of K, Na accumulation are not conducive to the formation of the biomass at the seeding stage of wheat. Total 141 QTLs distributed on all the 21 chromosomes are detected for the tested 20 traits. Fourteen important QTL clusters which including 104 QTLs are identified, and in which, 11 QTL clusters are colocated with K and Na related traits. Eight high frequency QTLs （RHF-QTLs） which could be detected simultaneously in different treatments are also identified in this experiment. These important QTL sites provide important references for further investigation of K and Na relations in plant nutrition and their genetic control.

关 键 词：小麦 重组自交系 钾 钠 QTL分析 

分 类 号：S512.1[农业科学—作物学]