HPLC-超滤离心法测定连翘酯苷复方脂质体包封率  

Determination of Entrapment Efficiency of Forsythiaside Compound Liposome by HPLC-Ulrafiltration

作　　者：祝文琪[1] 徐卫康[1] 侯晓林[1] 陆彦[1] 吴国娟[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《中国现代应用药学》2013年第9期1001-1004,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金项目(31172362);北京市自然科学基金项目(5102014)

摘　　要：目的 建立一种快速、准确测定连翘酯苷复方脂质体包封率的方法。方法采用逆向蒸发法制备连翘酯苷复方脂质体，建立检测连翘酯苷复方含量的高效液相色谱条件，用超滤离心法处理复方脂质体，测定连翘酯苷复方脂质体的包封率。结果用连翘酯苷和绿原酸对照品建立标准曲线，在0．78125-50μg-mL-1内线性关系良好，采用超滤离心法处理样品后连续3次测定包封率，平均包封率为92．38％和44．72％，其RSD分别为2．76％和2．35％。结论HPLC．超滤离心法可准确、方便的测定连翘酯苷复方脂质体的包封率。OBJECTIVE To establish a method for determining entrapment efficiency of forsythiaside compound liposome. METHODS The liposome were prepared by reverse evaporation method, and treated with ultrafiltration. HPLC was used to detect the content of drug in the forsythiaside compound liposome. RESULTS The standard curve for forsythiaside and chlorogenic acid was linear in the concentration range of 0.78 125-50 μg.mL-1. The mean value entrapment efficiency of forsythiaside and chlorgenic acid in the three samples were 92.38% and 44.72% with RSD of 2.76% and 2.35%. CONCLUSION The entrapment efficiency of forsythiaside compound liposome can be detenninated simply and quickly by the HPLC-ultrafiltration.

关 键 词：超滤离心法 高效液相色谱法 连翘酯苷 脂质体 包封率 

分 类 号：R917.101[医药卫生—药物分析学]