咳尔康口服液对哮喘模型大鼠NO、iNOS及支气管平滑肌炎症反应的影响  

Effects of Keerkang Oral Liquid on NO,iNOS and Bronchial Smooth Muscle Inflammation in Asthmatic Model Rats

作　　者：李厚忠[1] 张羽飞[2] 齐敏[3] 李孟全[2] 

机构地区：[1]牡丹江医学院药学院,黑龙江牡丹江157011 [2]牡丹江医学院医药研究中心,黑龙江牡丹江157011 [3]牡丹江医学院附属第二医院,黑龙江牡丹江157011

出　　处：《中国药房》2013年第39期3660-3662,共3页China Pharmacy

基　　金：黑龙江省卫生厅科研课题(No.2010-233);黑龙江省中医药科研项目(No.ZHY10-W73)

摘　　要：目的:研究咳尔康口服液对哮喘模型大鼠一氧化氮(NO)含量和诱导型一氧化氮合酶(iNOS)活性与支气管平滑肌炎症细胞浸润程度的影响。方法:通过卵蛋白法复制大鼠哮喘模型。40只大鼠均分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、醋酸泼尼松(10 mg/kg)组、咳尔康(8.3 ml/kg)组。采用硝酸还原酶法测定大鼠血清与肺组织中NO含量,酶联免疫法测定iNOS活力,HE染色切片半定量评定支气管周围炎症细胞浸润程度。结果:与正常对照组比较,模型组大鼠血清、肺组织中NO含量显著增加,iNOS活性显著增强,大鼠支气管平滑肌炎症细胞浸润程度加重(P<0.05);与模型组比较,咳尔康组大鼠血清、肺组织中NO含量显著降低,iNOS活性显著减弱(P<0.05),大鼠支气管平滑肌炎症细胞浸润程度明显减轻(P<0.01)。结论:咳尔康口服液可降低哮喘模型大鼠NO含量和iNOS活性,同时可以减轻支气管平滑肌的炎症反应,可能对哮喘有一定的防治作用。OBJECTIVE： To study the effects of Keerkang oral liquid on the activities of NO and iNOS and the inflammatory cell infiltration of bronchial smooth muscle in asthmatic model rats. METHODS： The asthmatic models were established by inject- ing ovalbumin （OVA）. 40 rats were divided into normal control group （constant volume of normal saline）, model group （constant volume of normal saline）, prednisone acetate group （10 mg/kg） and Keerkang group （8.3 ml/kg）. The level of NO and the activity of iNOS were detected in rat serum by nitrate reductase method and enzyme linked immune method respectively, and the inflamma- tory cell infiltration of bronchial smooth muscle was evaluated by HE staining. RESULTS ： Compared with normal control group, the levels of NO and the activity of iNOS in serum and lung tissue were increased significantly. The inflammatory cell infiltration of bronchial smooth muscle in rats was aggravated（P〈0.05） ;Compared with model group, the levels of NO and the activity of iNOS in serum and lung tissue were decreased significantly（P〈0.05）. The inflammatory cell infiltration of bronchial smooth muscle in rats was relived significantly（P〈0.01）. CONCLUSIONS： Keerkang oral liquid could decrease the level of NO and the activity of iNOS and relieve inflammation response of bronchial smooth muscle in asthmatic model rats. So it might have certain preventive and therapeutic effect on asthma.

关 键 词：咳尔康口服液 哮喘 一氧化氮 诱导型一氧化氮合酶 

分 类 号：R285[医药卫生—中药学] R99[医药卫生—中医学]